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- Identify and name the disease in which the patient’s cells lose the property of contact inhibition. State its possible causes and explain any three methods to accurately detect the pathological and physiological changes that take place due to the disease in living tissues.Use the following information to answer the next question. Human genes were integrated into the chromosomes of pig sperm using the procedure of sperm-mediated gene transfer (SMGT). In the first successful SMGT procedure, scientists used an enzyme to integrate three human genes into the chromosomes of pig sperm. Approximately 90% of the resulting pig embryos carried all three human genes in very cell. The presence of the human genes in every cell of a pig embryo is evidence that the genes went through Select one: a. mRNA transcription b. DNA replication c. mRNA translation d. DNA transcriptionCompare and contrast the method for determining the minimum inhibitory concentration (MIC) with the Kirby-Bauer disc diffusion test.
- Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) Egg whites contain about 88.5% water and about 10% proteins, from which ovalbumin is the major component. The eggs were cracked open, and the egg whites were separated carefully from the yolk. Stirring of the egg whites was done to break up the membrane. This was followed by filtration through cheesecloth, where a clear filtrate was obtained. About 40.0 mL of the filtered undiluted egg white was measured, and 0.10 mL of 1 M acetic acid was slowly added for every 1.0 mL of egg white with constant stirring. A turbid mixture with few amounts of white precipitate may be expected to form. These may be removed by filtering the mixture using a cheesecloth. What is the significance of adding 1 M acetic acid to the filtered egg white?Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation(Salting Out) Egg whites contain about 88.5% water and about 10% proteins, from which ovalbumin is the major component. The eggs were cracked open, and the egg whites were separated carefully from the yolk. Stirring of the egg whites was done to break up the membrane. This was followed by filtration through cheesecloth, where a clear filtrate was obtained. About 40.0 mL of the filtered undiluted egg white was measured, and 0.10 mL of 1 M acetic acid was slowly added for every 1.0 mL of egg white with constant stirring. A turbid mixture with few amounts of white precipitate may be expected to form. These may be removed by filtering the mixture using a cheesecloth After filtration, 30.0 mL of the filtrate was obtained, and the solution was brought from 0% to 40% saturation by adding the required amount of powdered ammonium sulfate, (NH4)2SO4 After adding ammonium sulfate, the mixture was allowed to stand…Crossmatching donor red cells for a patient involves combining: Question 2 options: A) 2 drops of donor red cells with 1 drop of patient plasma B) 2 drops of patient red cells with 2 drops of patient plasma C) 1 drop of donor red cells with 2 drops of patient plasma D) 1 drop of donor red cells with 1 drop of patient plasma
- describe the steps involved in primary cell dissociation protocol, assessing cell viability and sub culturing adherent cells with trypsin.A nuclear power plant employee, 20 years old, was entered the hospital with a diagnosis of "acute radiation sickness". In the analysis of blood, a decrease of all the blood elements was found. Questions: 1. What is the cause of the blood cell damage? 2. What other causes can lead to cell damage? 3. What mechanism of cell damage is the leading one in this case? 4. List the other possible mechanisms of cell damage. 5. Is it possible to restore the number of blood cells in this case? If so, how? 6. What compensatory mechanisms during cell damage do you know? 7. Indicate the principles of correction of cell damage.Identify three other methods commonly used to visualize lipids on a TLC plate. Specify the type or class of lipids that are detected using these methods.
- Give the color reaction in Wright's stain and the function of these cells. CELL COLOR REACTION IN FUNCTION WRIGHT'S STAIN RED BLOOD CELL NEUTROPHIL NUCLEUS: CYTOPLASM/GRANULES: EOSINOPHIL NUCLEUS: CYTOPLASM/GRANULES: BASOPHIL NUCLEUS: CYTOPLASM/GRANULES: LYMPHOCYTE NUCLEUS: CYTOPLASM/GRANULES: MONOCYTE NUCLEUS: CYTOPLASM/GRANULES: PLATELET CYTOPLASM/GRANULES:17) Sort the below given biomaterials -tissue interaction happenings according to occurrence time-line . Cell Response Protein adsorption on biomaterial Tissue Remodeling lon release from material Cell-Cell İnteraction Wear /degradation from material 18) The below given biomaterial characteristics may affect the overall in vitro/in vivo responses of the medical device EXCEPT a) Quantity and quality of material b) Degradation Products c) Physico-chemical properties of material d) Additives and contaminant, residuesName the tests we used to test for marcomolecules.