Describe what the plate in each scenario looks like: A. Bacteria grows on mannitol salt agar and is negative for mannitol fermentation B. Bacteria grows on EMB agar and is positive for lactose fermentation C. Bacteria grows on MacConkey agar and is negative for lactose fermentation
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- For each of the following situations, describe how bacteria on these growth. media would appear on the plate: A. On MacConkey agar, a bacterium that grows on the medium but is negative for lactose fermentation B. On EMB agar, a bacterium that grows on the medium but is positive for lactose fermentation C. On mannitol salt agar, a bacterium that failed to grow on the medium D. On mannitol salt agar, a bacterium that grew on the medium and fermented mannitolDescribe how a Triple Sugar Iron agar tube would appear if the following situation was true. Please note that all items in the following list are occurring in the same tube of agar. 1. Positive for glucose fermentation 2. Negative for lactose and sucrose fermentation 3. Positive for H2S production 4. Negative for gas productionWhich of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.
- Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli b. Pseudomonas aeruginosBelow is an image of 3 different bacteria, letter labeld, growing on MacConkey Agar. Note that bacteria such as E. coli use mixed acid fermentation, with very high acid production which will cause a colored precipitation around colonies on MacConkey agar. MacConkey Agar CThe following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? TAW ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar lactose metabolism
- Why is Phenol red being utilized in MacConkey agar? Choose among the choices and further explain it.A. Inhibit microscopic organismsB. Detect the production of gas by-productsC. Conduct the sulfate reduction testD. Detect the acid reaction of lactose fermentationIn spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.What is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in only one? Which sugars are typically tested in the phenol red broth? Can the phenol red test be also used to determine if a certain bacteria can metabolize various carbohydrates? Explain how conducting the MR/VP test too soon in a culture’s growth cycle can lead to false negatives? Generally, the color change we see in these tests is a result of what change in the media? Explain. What is the MR-VP results for coli and E. aerogenes?
- Microorganisms are used in the production of a wide variety of fermented products. a) Using an example of a fermented product, explain the role of microorganisms in the production of this product. b) Explain why it is very important to control the quantity of food sources that these microbes metabolize?Why is the batch bioreactor is suitable in milk fermentation?Agar is a solidifying agent used in media preparation.a. What is its origin? b. What makes it ideal for cultivation of microbes? c. How and why does the agar concentration in semisolid media differ from conventional solid media?