Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger sequencing (ddNTP's): a. Lane 1: A pre-made ladder with the following molecular weights: 2, 5, and 9 b.p. Draw and arrow and label each band for your ladder. b. Lane 2: PCR of sample using dGTP, dATP, dCTP, and dTTP c. Lane 3: PCR of sample using ddGTP, dATP, dCTP, and dTTP d. Lane 4: PCR of sample using dGTP, ddATP, dCTP, and dTTP e. Lane 5: PCR of sample using ddGTP, ddATP, ddCTP, and ddTTP

Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger sequencing (ddNTP's): a. Lane 1: A pre-made ladder with the following molecular weights: 2, 5, and 9 b.p. Draw and arrow and label each band for your ladder. b. Lane 2: PCR of sample using dGTP, dATP, dCTP, and dTTP c. Lane 3: PCR of sample using ddGTP, dATP, dCTP, and dTTP d. Lane 4: PCR of sample using dGTP, ddATP, dCTP, and dTTP e. Lane 5: PCR of sample using ddGTP, ddATP, ddCTP, and ddTTP

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

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