Colorimetric reagents. Proteins will intensely absorb 280 nm light even in the absence of a colorimetric reagent (See figure). Why is it advantageous to use the Bradford reagent to r protein concentration rather than just measuring protein absorbance (280 nm) directly?

Colorimetric reagents. Proteins will intensely absorb 280 nm light even in the absence of a colorimetric reagent (See figure). Why is it advantageous to use the Bradford reagent to r protein concentration rather than just measuring protein absorbance (280 nm) directly?

Organic Chemistry

8th Edition

ISBN:9781305580350

Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions

Section: Chapter Questions

Problem 20.25P

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Colorimetric reagents. Proteins will intensely absorb 280 nm light even in the absence of a
colorimetric reagent (See figure). Why is it advantageous to use the Bradford reagent to r
protein concentration rather than just measuring protein absorbance (280 nm) directly?

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