Q: nd cell line same
A: Cell culture is that the culture of cells is directly isolated from parental tissue of interest;…
Q: Temperature plays a very important role in the growth of fungi, bacteria, and other microorganisms.…
A: Milk serves as a nutritious source of food for humans. Milk favors the growth of mesophilic microbes…
Q: what is the name of the first step of cellluler respritation
A: Cellular respiration is a metabolic pathway that breaks down glucose and produces ATP.
Q: What are some reasons a Gram positive cell might appear Gram negative? What color would a Gram…
A: Gram-positive bacteria have a thick peptidoglycan layer and no outer lipid membrane whilst…
Q: atch the descriptions given below to either the capsule or slime layer. Capsule Slime layer easily…
A: Bacteria are microscopic single-celled prokaryotes that thrive in diverse environmental conditions.…
Q: Could you spread plate in the usual Petri dish a 10mL inoculum? Why or why not?
A: The spread plate technique involves spreading a small amount of bacteria suspended in a solution…
Q: How does a cell transform nutrients into the fundamental structures required for its survival (cell…
A: Cells need nutrients to get the necessary elements. Different food is the source of different…
Q: Why are disease causing flagellate considered dangerous?
A:
Q: You are doing a viable cell count for a culture of E. coli you are growing in a flask. The volume of…
A: Viable cell means living cells whereas non-viable cells are dead cells. Viable cells of bacteria…
Q: E. coli cells have peritrichous flagella. What does this mean?
A: E.coli. is a type of bacterium. Bacteria are unicellular and prokaryotic organisms that live in a…
Q: What is secondary cell culture?
A: Secondary culture is a subculture of the primary culture. All the cells grown in the Petri dish…
Q: (this is a potato/starch under microscope) 1. label the parts of the cell and amyloplastids 2. what…
A: Plants produce glucose by the process of photosynthesis and they are called as autotrophs.…
Q: Lipopolysaccharide (LPS) is a major feature of which cell wall? O Fungal O Viral O Gram-positive…
A: Lipopolysaccharide are large molecules consisting of a lipid and a polysaccharide. They are found as…
Q: Find and label the cell wall, cytoplasm, vacuole bounded by tonoplast, nucleus (if visible) of this…
A: Plasmolysis is defined as the process of contraction or shrinkage of a plant cell protoplasm and is…
Q: Picture Analysis 2: Give your comprehensive analysis of the image below: Chame Carrier
A:
Q: cysts best fit the following descriptions ? Nucleus: 1 in mature cyst Peripheral chromatin: None…
A: * Endolimax Nana is nonpathogenic present in lumen of the large intestine in the humans…
Q: Organelle mixture 1.09 1.11 Centrifuge 1.15 2 - 1.19 1.22 1.25 Which fraction would you take to…
A: Sucrose density gradient centrifugation is a technique to separate subcellular organelles and for…
Q: What are types of cell culture media?
A: A cell culture medium is solid or liquid in nature. It is a mixture of all necessary nutrients…
Q: Which below is/ are acellular? (best answer)
A: Cellular organisms are characterized by the are organisms characterized by the presence of cell…
Q: During Boxidation, which of the below can only be formed in the presence of H0? OA CH,…
A: Lipids are macromolecules that are mainly water-insoluble molecules. They are not polymers like…
Q: How to prevent MDCK cells from forming cysts in culture?
A: MDCK is also known as Madin-Darby Canine Kidney. The cells are a type of model mammalian cell used…
Q: What conditions may cause a false positive test for phagocytic engulfment? How could these factors…
A: Phagocytosis is a cellular event that is essential for the clearance of dead cells from the system…
Q: the morphology of the crushed nodules under microscopes
A: Active nodules are red coloured inside Actively N‐fixing nodules contain a pigmented protein…
Q: A 73 year old woman is admitted to hospital for treatment of an abscess from staph. It was necessary…
A: Abscess is the confined pocket of pus that has built within the organs or tissues of the body.…
Q: meaning of Inoculate
A: MICROBIAL GROWTH MEDIA- It is the culture media that serves as a nutrient medium for the growth of a…
Q: How does amoeba ingest a food particle through the process of phagocytosis?
A: Amoeba An amoeba is an eukaryotic unicellular organism that has ability to change shape.
Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…
A: Cell organelles extraction The process to obtain cell organelles from the cell.
Q: Which type of carbohydrate will be the most effective to increase cellular respiration rate in the…
A: According to their requirement for oxygen, yeasts are classified as facultative anaerobes. This…
Q: Bacterial cells move with the use of _________________.
A: Bacteria are the most important microorganisms to the food processor. Some bacteria are beneficial…
Q: What are the 5 stages of the lytic cycle?
A:
Q: How should agar plates be incubated? Why?
A: Incubating the plates to stimulate the growth of microbes is a crucial step in any microbiology…
Q: 11. Trypticase Soy Agar • A. Defined • B. Complex • C. Differential • D. Selective • E.…
A: Trypticase soy agar or TSA with agar is a growth medium that is used to culture bacteria. TSA is…
Q: Onion Draw 4 cells under LPO and I cell under HPO. Label the visible parts.
A: 1. In microscopes, LPO means Low Power Objective and the power of the LPO is 10X. 2. HPO means…
Q: What are the steps of the lytic cycle? How do the lytic and lysogenic cycles differ? How are they…
A: Virus is a acellular structure which remain inactive outside the host but active inside the host .…
Q: Contrast the overall cell wall compositions of Gram positive and Gram negative bacteria, and go…
A: The bacteria are classified according to their cell wall composition into Gram positive and Gram…
Q: What is the percentage of viable cells shown in the image? 10 O
A: Answering by considering the dye used is trypan blue Cell viability assays are used to measure the…
Q: Describe bacterial sporulation in your own words.
A: Bacterial sporulation is a method of evading unfavourable environmental conditions. But some regard…
Q: In monolayer cell culture conditions contact inhibition occurs when the following conditions are…
A: Contact inhibition is a process of arresting cell growth. When cells come in contact with each other…
Q: Why are agar plates incubated upside down?
A: The Petri dish has existed for over a decade, whereas simple, cell-growing equipment remains…
Q: A right-handed pressed hands onto agar plates. There are four plates total--left hand palm, left…
A: The skin harbours a variety of bacterial strain on certain regions of the body. The bacterial niche…
Q: Which of the following blocks cell wall synthesis? O ciprofloxacin erythromycin rifampin mebendazole…
A: Cell wall is the outermost layer of the microbes. The cell wall inhibitors are the class of drugs…
Q: The following image shows part of counting chamber you used to estimate cell viability for yeast…
A: For the calculation of cell viability:- 1. All the dead cells and the living cells are added for…
Q: commonly used to stain cytoplasm granules blue
A: On the basis of staining the microbes can also be distinguished as gram-positive and gram-negative…
Q: What is the shape of the following cells? spirochete cocci spirillum…
A: INTRODUCTION The shape of the bacteria in the picture is given below.
Q: How does one isolate Aconitase from a tissue sample? From the start of the tissue sampling to…
A: Aconitase is an iron-sulfur protein-containing enzyme that catalyzes citrate to isocitrate via cis…
Q: Plasmolysis in plant cell Describe the cell in 0.1 % Salt Solution and explain the process.…
A: Solution is formed by the combination of solute molecules and solvent medium . Solute is salt /…
Q: Actinomycetes are thin, beaded, branching, positive rods that are aerobic. Nocardia spp. and…
A: **As per our guideline, we are supposed to answer only one question at a time. Please resubmit the…
Q: What are the differences between “primary cell culture” and “continuous cell lines”?
A: Primary cell culture is the culture of cells directly isolated from parental tissue of interest.…
- What is the cell lysate?
- What is the purpose of the cheesecloth?
Step by step
Solved in 2 steps
- Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 1 (1) (ii) 3 (iii) 2 (iv) 4 Note: Count cells on the four labelled squares. Include cells touching the line on top and left. 18P 06. ¿66 Ⓡ Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.What is the purpose of Punnet square?How is the precipitation of proteins by alcohol applied in the fixing of tissues for histological purposes?
- Why and How is brick weathe resistant?What is the effect of trypsin EDTA solution on monolayer cell culture preparation?65. Destruction of the group of cells identified by the arrows in the photomicrograph shown is most likely to result in which of the following conditions? A) Anemia B) Cirrhosis C) Impotence D) Type 1 diabetes mellitus E) Ulcer