Buffers are aqueous solutions that tend to resist changes in pH when small amounts of strong acid (H+) or base (OH-) are added. Compare the changes in the pH of the resulting solution when you add 0.1 M NaOH in (a) distilled water and (b) bicarbonate solution. A) (a) and (b) solutions will have the same pH. pH change in (b) will be higher than in (a). C) No changes in pH of both solutions. D) pH change in (a) will be higher than in (b).
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- What is the pH of the following buffer mixtures? (a) 100 mL 1 M acetic acid plus 100 mL 0.5 M sodium acetate (b) 250 mL 0.3 M phosphoric acid plus 250 mL 0.8 M KH2PO4Benzalkonium chloride 17% w/v . . . . . qs Water qs . . . . . . . . . . . . . . . . . . . . . . 120 mL M. ft . solution such that 2 tbsp added to a pint of water will yield a 1:750 solution. How many milliliters of the 17% w/v stock solution will be needed to prepare the 120 mL? a 2.68 mL b 7.90 mL c 15.80 mL d 20.40 mL e 6.28 mL4.2 (b) Using the equation below, explain what happens to maintain the pH of the solution: A (aq) H(aq) HA (aq) (i) when an acid is added (ii) when a base is added (iii) Give one example each of a buffer in both living and non-living systems +
- You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M KOH solution. If you have 730 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.43? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.Calculate the theoretical pH of a Tris buffer (pH 8.0) at 0 ºC. Assume that room temperature is 22 ºCIf 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.
- (2) Tris buffer is an organic buffer that is commonly used in Biochemistry experiments in lieu of inorganic buffer. Discuss the structure, buffering properties, and advantages of using Tris buffer. Cite 2-3 references properly.Calculate the pH of the resulting solution if 23.0 mL of 0.230 M HCl(aq) is added to 33.0 mL of 0.230 M NaOH(aq). pH = Calculate the pH of the resulting solution if 23.0 mL of 0.230 M HCl(aq) is added to 13.0 mL of 0.330 M NaOH(aq). pH=Calculate the pH of a buffer that contains 0.75 M acetic acid and 0.35 M acetate ion in 1 L solution. What will the pH of the buffer be upon the addition of 100.0 mL of 1.0 M HCI? (pKa of acetic acid 4.76)
- Determine the weight/volume of the chemicals needed to prepare the following solutions: a) 100 ml of 0.9% (w/v) saline (NaCl) b) 30 ml of 50% glycerol (v/v) c) Electrophoresis requires TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. TAE is normally made as a 50X concentrated stock. Provide a recipe to make 40 ml of 50X TAE. The recipe for one liter of 50X TAE is as follows: 242g Tris base, 57.1 ml glacial acetic acid, 100 ml 0.5 M EDTAHow to create a 750 uL of XS buffer (with compositions 1% potassium ethyl xan- thogenate; 100 mM Tris-HCl, pH 7.4; 1% sodium dodecylsulfate; 800 mM ammonium acetate)? Show calculationsWhat is the pH of a buffer that contains 0.9 M ammonia and 0.3 M ammonium ion? (Ka for ammonium ion = 5.8 x 10-10