Briefly explain the principle of Bradford Method of Total Protein Estimation
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Briefly explain the principle of Bradford Method of Total Protein Estimation.
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- Discuss the advantages and disadvantages of Protein efficiency ratio(PER)Give two benefits of utilizing the biuret reaction to assess protein concentration over directly measuring protein absorbance at 280 nm.Sketch the appearance after visualization of a protein mixture containing the seven proteins (fibrinogen, y-globulin, collagen, ovalbumin, myoglobin, hemoglobin, insulin) when subjected to two-dimensional (2D) gel electrophoresis.
- Discuss the functions and components of Biuret dye in protein content determination. Identify two other methods of protein determination and differentiate them from Biuret method. Why is BSA used as standard for protein content determination? Can other proteins be used instead?(Formulation using Pearson Square Method) Using Pearson Square Method, formulate 50 kilograms of a ration with 18% CP using soybean meal and yellow corn. How much of each ingredient (in kg) should be used in order to satisfy the protein requirement of the animal?i. What are the advantages of biological value of protein method compared to N-balance and chemical score? ii. A bull consumes 7 kg DM of feed containing 60 g N/kg. The bull excretes 120 g N in the faeces and 50 g N in the urine. The feacal and urinary N had 20 g metabolic feacal nitrogen (MFN) and 6 g endogenous urinary nitrogen (EUN) respectively. Calculate the BV of the protein in the diet.
- Table 5: Effect of Dietary Fiber on Bile Acid Adsorption Optical Density (OD) of Colored Filtrate (Reflects Amount of Bile Acid in Filtrate) 1- OD (Reflects Amount of Bile Acid Adsorbed by Food Particles) Fiber- Fiber- Fiber- Fiber- Bile Acid With Buffer Solution (No Fibers) Bile Acid With Buffer Standard Standard Rich Rich Standard Standard Rich Rich Meal Meal Meal Meal Мeal Meal Мeal Meal With Bile Experimental Solution (No Trials Without With Bile Without Without With Bile Without With Bile Fibers) Bile Acid Bile Acid Bile Acid Bile Acid 1 1 0.55 0.4 0.00 2 0.61 0.4 3 1 0.52 0.32 Average Equations (f,) Use these formulas for making your calculations. Round your answer to the nearest tenth. Averages Add the values from the three trials (or subjects); divide by three. Index reflecting the level of bile acid adsorption This index is equal to 1- OD, where OD is optical density of the colorized supernatant of mixtures of the studied food. This index reflects the amount of bile acid…Which technique will be most suitable for desalting the protein or analyzing the actual dimensional (D) native form and what would be the advantages of this selection? Discuss two to three supportive evidence of the technique application.In the ESTIMATION OF TOTAL SERUM CHOLESTEROL using ZAK AND HENLY’S METHOD, explain why should the serum stay clear? What are the implications of using a turbid serum sample?
- Table 5: Effect of Dietary Fiber on Bile Acid Adsorption Optical Density (OD) of Colored Filtrate (Reflects Amount of Bile Acid in Filtrate) 1- OD (Reflects Amount of Bile Acid Adsorbed by Food Particles) 000 Fiber- Fiber- Fiber- Fiber- Bile Acid Standard Standard Rich Rich Bile Acid Standard Standard Rich Rich With Buffer Meal Meal Meal Meal With Buffer Meal Meal Meal Meal Experimental Solution (No Trials With Bile Solution (No Fibers) Without Without With Bile Without With Bile Without With Bile Fibers) Bile Acid Bile Acid Bile Acid Bile Acid 1 1 0.55 0.4 0.00 2 1 0.61 0.4 3 1 0.52 0.32 Average 0.00 0.00 0.00 0.00 Equations (fx) Use these formulas for making your calculations. Round your answer to the nearest tenth. Averages Add the values from the three trials (or subjects); divide by three. Index reflecting the level of bile acid adsorption This index is equal to 1 - OD, where OD is optical density of the colorized supernatant of mixtures of the studied food. This index reflects…pls explain Increasing the saturation of the ammonium sulfate is a prerequisite in isolating a target protein that is rich in Cys and Tyr residues. Which of the following techniques should be considered in accurately quantifying the isolated protein?I. Running the isolated protein in a dialysis or GFC set up.II. Using Biuret or BCA assay as the colorimetric quantitation method.III. Using Bradford or Lowry assay as the colorimetric quantitation method.A. I onlyB. II onlyC. I and IIID. I, II and III. Bradford Assay is most suitable to use when the extraction buffer is below the target protein’s pI. This is so because the protein would be morea. Positively charged allowing the CBB G-250 dye to bind via its sulfonate groups.b. Negatively charged allowing the CBB G-250 dye to bind via its sulfonate groups.c. Neutrally charged allowing the CBB G-250 dye to bind via its sulfonate groups.d. Zwitterionic allowing the CBB G-250 dye to bind via its sulfonate groups.Give two advantages and two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm