Bdellovibrio would be great to use to treat all human pathogens, but it cannot be used as biological control for all bacteria that would be pathogenic to humans. Why not?
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Pathogenicity
Infection and Transmission
The infections are generated by the pathogenic organisms present in the environment. They maintain the capacity to invade a host body and establish colonies. A disease caused by such infectious agents is called a communicable disease or transmissible disease. These diseases spread through diverse means including blood, food, water, air, or vectors.
Bdellovibrio would be great to use to treat all human pathogens, but it cannot be used as biological control for all bacteria that would be pathogenic to humans. Why not?
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- Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?How would you grow a streptomycin-resistant strain E. coli bacteria was spread on both agar plates and incubated for 24 hours. Neg. reptomycia Po. If you wanted to grow up a large quantity of streptomycin resistant E. coli, what would you do next? Multiple Choice n:-. - Next > 13 of 20 ENG < Prev E O A 4) INTL search
- You are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A growing culture of B. cereus is inoculated with your bacteriophage. The day before you tested the bacteria and the phage batch and they both behaved as expected. You made sure that all containers are labeled appropriately. Immediately after inoculation, you take a sample but are unable to detect any bacteriophage in the media. What is the most likely explanation? O You used the wrong bacteria or the wrong bacteriophage. There is an issue with the phage batch. O There is a problem with your bacterial culture of B. cereus. O It is too soon. The bacteriophages are still replicating and assembling inside the bacterial cells and are therefore not detectable in the growth media.Katelyn had been working for Dr. Johnson for a month, and while she had become quite good at measuring inhibitionzones, she didn’t know why she was doing all this work. She had gotten very curious after she began doing all themeasurements on a new set of antibiotics. # is experiment involved infecting mice with MRSA and tracking how theMRSA grew over time.Data were collected by counting the cells of MRSA taken from $ uid samples from the mice. # e cells were measuredby taking one gram of the $ uid and spreading it over plates, but now Katelyn counted the colonies that grew on theplate after 24 hours. Because there were so many, she actually measured the colonies as “log CFU/g.” A CFU is acolony forming unit, or essentially a cell that will divide into a colony that can be seen. Because there can be so many,Katelyn measured them on a logarithmic (log) scale. # e raw data in her lab notebook looked like the following:Table 1. E% ect of treatment on MRSA in mice after 24 hours of drug…Which of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plate
- Phage therapy can be used to drive bacteria to evolve to become less____________ to humans.Which of the following experimental techniques was used by Hershey and Chase to establish the genetic material of phage? * None of the above X-ray Isotope labelling Chromatography UltracentrifugationTRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?
- What is the benefit of using LB (Lysogeny broth) medium for the growth of E. coli, Bacillus subtilis and Pseudomonas putida? Why ?You are working for the CDC studying outbreaks of foodborne infectious disease. Your latest case is an incidence of food poisoning in a fast food restaurant. 5 people have been hospitalized for food poisoning, so you are working with that hospital to identify the source of the outbreak. Hospital clinicians have taken samples from each of the 5 patients for culturing. Each bacterial culture grew one likely microbe, each labelled P1, P2, P3, P4, and P5. All 5 isolates appear to have the same colony morphology on the plates, and all appear to be Gram-negative rods 1 micron wide and 3 microns long under the microscope. In addition, all 5 isolates appear to perform the same on an API 20E test strip that tests bacteria for 20 biochemical abilities. No other testing has been done yet. How would you best describe your set of isolates at this moment? O Each isolate is the same strain, of a different species O Each isolate is a different strain, of different species O Each isolate is a different…Suppose your professor handed you a test tube with 2.0 mL of M. foliorum infected with bacteriophage T4 in it and told you to make a 10-2 dilution of the entire culture. Explain how you would do this. Show your calculations.