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Below is the titration of histidine. Calculate the average charge of histidine at pH 6.50. ÇOON Histidine 10 pK, - 9.17 pl= 7.59 6.0 PH pK, = 1.82 1.0 2.0 3.0 OH" (equivalents)

Below is the titration of histidine. Calculate the average charge of histidine at pH 6.50. ÇOON Histidine 10 pK, - 9.17 pl= 7.59 6.0 PH pK, = 1.82 1.0 2.0 3.0 OH" (equivalents)

Anatomy & Physiology
Anatomy & Physiology
1st Edition
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Chapter2: The Chemical Level Of Organization
Section: Chapter Questions
Problem 31RQ: Uracil ________. contains nitrogen is a pyrimidine is found in RNA all of the above
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6. Below is the titration of histidine. Calculate the average charge of histidine at pH 6.50. соон Ç00 çoo HN-CH HN-CH HN-CH CH, H CH, H CH, H CH, H CH CH pK. CH pk. pK. HN +2 +1 pK, = Histidine 10 9.17 8 pK = 6.0 pl = 7.59 6 PH pk, = 1.82 2.. 1.0 2.0 3.0 OH (equivalents) 7. Consider the following reaction Malate + NAD+ → oxaloacetate + NADH + H* AG" = 29.7 kJ/mol a. The concentration of NAD+ and NADH are 20 mM and 0.3 mM, respectively. If malate is 0.4 mM, what is the maximum concentration of oxaloacetate needed to make the reaction exergonic at pH 7.0 and 37 °C? b. Which species is oxidized the malate of the oxaloacetate? c. Calculate AGO' using the reduction potentials look in your text for the values. How does it compare to AG" = 29.7 kJ/mol? AG" = -nFAE°r = -nF|E (r accepier) - E° (e donor)
Transcribed Image Text:6. Below is the titration of histidine. Calculate the average charge of histidine at pH 6.50. соон Ç00 çoo HN-CH HN-CH HN-CH CH, H CH, H CH, H CH, H CH CH pK. CH pk. pK. HN +2 +1 pK, = Histidine 10 9.17 8 pK = 6.0 pl = 7.59 6 PH pk, = 1.82 2.. 1.0 2.0 3.0 OH (equivalents) 7. Consider the following reaction Malate + NAD+ → oxaloacetate + NADH + H* AG" = 29.7 kJ/mol a. The concentration of NAD+ and NADH are 20 mM and 0.3 mM, respectively. If malate is 0.4 mM, what is the maximum concentration of oxaloacetate needed to make the reaction exergonic at pH 7.0 and 37 °C? b. Which species is oxidized the malate of the oxaloacetate? c. Calculate AGO' using the reduction potentials look in your text for the values. How does it compare to AG" = 29.7 kJ/mol? AG" = -nFAE°r = -nF|E (r accepier) - E° (e donor)
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