ATP stock (50 µM): Make pre-dilutions with 20, 15, 10, 5, 2,5 and 1,25 µM ATP (1000 μL of each) Inhibitors Rotenone Oligomycin CCCP KCN Antimycin A Calculate the needed volumen of the standards! Inhibitors of ETC Stock concentations Final concentrations (in 250 μL) 400 µg/ml in DMSO 1250 µg/ml in EtOH 1000 µg/ml in DMSO 400 mM in Tris-buffer 1 mg/ml in DMSO Calculate the needed volumen! 8 µg/ml 25 µg/ml 20 µg/ml 8 mM 20 µg/ml
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- Calculate the concentration of p-aminophenol control to match the 0.075 % w/w limit in 200 mg/10 ml Your Answer: 0.0015% show workNational Board of Medical Examiners Biochemistry Mark 36. In the presence of a metabolite (X), 6-phosphofructokinase is assayed at a fixed concentration of ATP and varying concentrations of fructose 6-phosphate. The resulting data are shown in the table. Fructose 6-phosphate (pM) 5 10 20 40 75 100 200 Velocity umoles/min 0.05 0.15 0.25 0.70 1.7 2.2 3.1 3.1 Velocity (+X) umoles/min 0.006 0.025 0. 10 0.35 1.03 16 2.9 3.1 400 Metabolite (X) is most likely which of the following substances? O A) ADP O B) AMP OC) CAMP D) Citric acid O E) Fructose-2,6-bisphosphateSubstrate Concentration (mol L1) Velocity (mM min-1) 2.500 0.588 1.000 0.500 0.714 0.417 0.526 0.370 0.250 0.256 Determine the values of Km and Vmax for the decarboxylation of a 훃-keto acid given the followingdata. You have to plot the graph by using excel and please include the scope of graph
- Table of caffeine standards concentration Sample Conc, ppm Std1 16 Std2 32 Std3 48 Std4 64 Std5 80 If the volume used to make 100 mL of std 1 is 2 uL what is the concentration in M used to make a standard calibration curve? The standards are going to be used to build calibration curve to analyze caffeine in an energy drink. If 500 mL of the energy drink has target of 400 mg caffeine, how will you prepare the sample if you need 10 mL for the analysis ? . Caffeine MM=194.19 g/mol.Arachidonic Rate of formation Rate of formation of PGG2 with 10 mg/ml acid (mM) of PGG2 (mM/min) ibuprofen (mM/min) 0.310 12.7 7.92 0.372 14.3 9.12 0.558 18.0 12.2 0.930 2.17 22.8 29.6 16.8 24.6 The kinetic data given in the table are for the reaction catalyzed by a mutant of prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. Vmax= TOOLS Km = x10x mM/min mM Publisher: WH FreWhat is time constant ? The steel ball bearings of 40 mm diameter and initially at uniform temperature of 600 °C are quenched in an oil bath maintained at 50 °C temperature. The heat transfer coefficient between the ball bearing and oil is 325 W/m2-K and the thermodynamics properties of the bearing can be taken as : k 45 W/m-K and thermal diffusivity a = 1.25 x 10-5 m/s. Determine : The time duration for which bearing must remain in oil to attain 225 °C temperature. b. The instantaneous heat transfer rate from the bearings when they are first immersed in oil and when they reach 225 °C.
- Chitinase is a protein that breaks down chitin, a primary component of the cell wall in fungi, scales in fish and exoskeletons of arthropods. The activity of chitinase extracted from a plant was shown to be optimum at pH 5. You were tasked to prepare 300 mL of 150 mM buffer solution for further analysis of the extracted chitinase. REAGENTS Ka 2.5M Acetic acid Solid NaOAc•3H2O [136.08g/mol] 1.76 x 10-5 2.5M NH3 Solid NH4Cl [53.49g/mol] 5.6 x 10-10 2.5M Lactic acid Solid sodium lactate [112.06g/mol] 4.0 x 10-5 5 M HCl 5M NaOH Pls show sol'ns 1. Given the following reagents, give the moles of each component (acid & base).2. What are the mass/volume of the components needed to prepare the buffer? 3. What will the pH of the buffer be if 1mL of 5 M NaOH was added?Computation: Ratio Strength, PPM, mg%Show your complete solution.1. How many grams of a 1:80 trituration of strychnine sulfate should be used for this prescription?RxStrychnine sulfate 45mgBelladonna extract 360mgSucrose 36gFt. chart; Div. in #XXXVIAmount of Strychnine in grams = ?(1) Enzy Matic, a Biochemistry student is studying the mode of inhibition of maleate to fumarase enzyme. Fumarase is the enzyme in the Kreb's cycle that converts fumarate to malate. He obtained the following kinetics data from her experiment: Velocity (mM/min) Inhibited 1.38 [S], mM Uninhibited 1.75 1.94 2.17 3.00 5.50 2.26 1.67 2.85 3.55 2.13 2.97 10.5 4.39 3.83
- Calculate the standard reduction of 3-phosphoglycerate using val ues from your book, and compare the reduction potential to others tabulated in the book.PRESSURE OF A WHAT IS THE asmoTIC SOLUTION OF BOVINE insuLIN (MOLAR MASS, 5700g mol - 1 ) aT 18 °C IF 100.0 ML OF THE SOLUTION conTains 0.103 a OF THE INSULIN?1000 Tablets 48, Kandivli Ind Estate, Mumbai 400 067, India by: Ipca Laboratories Limited Jacksonville, FL 32257 USA Ranbaxy Pharmaceuticals Inc. Manufactured for Rx only LOT: EXP: 100 mg TABLETS, USP ALLOPURINOL N Each scored tablet contains: Allopurinol, USP........100 mg Store at 20° to 25°C (68° to 77° F) [See USP Controlled Room Temperature]. Dispense in tight, light-resistant container as defined in the USP. USUAL DOSE: One tablet daily. For indications, dosage, precautions, etc., see accompanying package insert. Code: DNH/DRUGS/NH/170 3 633041153910 NDC 63304-539-10 Non varnish area R RANBAXY LBZTL6 0311 Expiration date Prescription warning Total amount in vial, packet, box Usual adult dose Dosage strength Drug form Lot number (control number) National Drug Code (N D C) # Manufacturer Brand name Generic name