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- Choose one specific way from each of the two methods of inhibiting the growth of microbes below. Describe the method, explain the mechanism of action of killing the microorganisms and give practical applications of your chosen ways. 1. Physical Methods - Heat, cold, dessication, radiation, ultrasonic waves, filtration, gaseous atmosphere 2. Chemical Methods- disinfectants, antisepticsВackground In order to determine whether a newly synthesized chemical might be a useful food preservative, the chemical was tested for its ability to inhibit bacterial growth. Control. 500 ml of cottage cheese was inoculated with 2 ml of a 24-hr culture of Pseudomonas aeruginosa and incubated at 25°C. Five hours after inoculation, a standard plate count showed there were 200 bacterial cells/ml in the cottage cheese. After 11 hours, 1600 cells/ml. After 21 hours, 50,000 cells/ml. After 27 hours, 400,000 cells/ml. Experiment. 500 ml of cottage cheese containing the preservative was inoculated with 2 ml of a 24-hr culture of P. aeruginosa. After 6 hours of incubation at 25°C, a standard plate count was performed. There were 700 bacterial cells/ml in the cottage cheese. After 14 hours, 11,000 cells/ml. After 20 hours, 90,000 cells/ml. After 28 hours, 1, 400, 000 cells/ml. Use the data and the semi-log graph paper to plot growth curves for the control and the experiment. Remember to label…(1) How did u measure the zone of inhibition (2) Which unit of measurement was used (3) For two organisms,choose two antimicrobial and explain whether the organism was susceptible, intermediate or resistant. Please make the answer as short as possible .Thanks
- Background: The following results were obtained from a broth dilution test for microbial susceptibility. What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?Define ‘thermal death point’ and ‘thermal death time’ How does the addition of salt or sugar help preserve food? Why were chemical disinfectants once commonly compared with phenol? When is using a broad-spectrum antimicrobial drug warranted?If lysozyme is added to a microbial culture. Predict what will be observed if the culture is affected by the action of lysozyme. A change in color will be visible in the tube. An increase in absorbance will be observed over time. A decrease in absorbance will be observed over time. An increase in turbidity will be observed over time.
- Compare and contrast antiseptic and disinfectant. What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can inoculation loops and needles be sterilized by autoclaving? Explain.EXPERIMENT:EFFECT OF CARBON SOURCES ON THE MICROBIAL GROWTH. 1.OBJECTIVE To study the effect of media components on microbial growth 2.PROCEDURES Preparation of fungal suspension inoculums Prepare the laminar cabinet for the inoculum preparation by applying normal aseptic techniques To a full-grown fungal Petri dish, add 25ml sterilized distilled water and rub the agar gently with glass rod Pour the suspension into an empty flask and continue previous steps to achieve your desired volume of inoculums suspension. Cotton-plugged and aluminium-wrapped the mouth of inoculum flask. For certain fungal species like Niger, the suspension from the agar should be filtered upon pouring into the inoculum’s flask. Preparation of salt media of different carbon sources. Prepare the Modified Czapek Dox media by weighing the following salts according to your desired working volume. KH2PO4 (1 g/L) NaNO3 (3 g/L) MgSO4.7H2O (0.5 g/L) Carbon source (30 g/L) KCl (0.5 g/L) 2.…A microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its growth. Three flasks of the same media have different amounts of NaCl added. Other growth conditions are identical, and the flasks inoculated at the same time. At regular intervals over a 3 hour period, culture samples are taken and turbidity is measured. Here are the absorbance values over time in a table and graphed. Absorbance (OD600) Time (min) 20% NaCl 3% NaCl 0% NaCl 0 0.05 0.05 0.05 30 0.06 0.05 0.05 60 0.075 0.06 0.05 90 0.11 0.07 0.049 120 0.25 0.1 0.048 150 0.5 0.105 0.055 180 0.7 0.11 0.055 210 0.99 0.15 0.06 240 1.22 0.18 0.055 270 1.35 0.19 0.06 300 1.5 0.2 0.06 330 1.6 0.25 0.062 360 1.7 0.3 0.06 Based on these data, which of the following can be determined about this bacterium? It is psychrophilic. It…
- Considering microbial identification methods, to which category would the following method belong based on what it measures? * Performing a hydrogen peroxide breakdown assay to see if a microbe produces the enzyme catalase Serological O Biochemical Genotypic PhenotypicIn this exercise, the action of bactericidins will be evaluated by __________. In this exercise, the action of bactericidins will be evaluated by __________. observing color change counting the number of colonies on agar viewing lysozyme activity measuring turbidity measuring microbial metabolic activityIf you have 4 compounds and you test them for antimicrobial activity using a disk diffusion assay and one is very active, one is moderately active, one is slightly active and one is not active at all, draw the disk, the zone of inhibition that you would see and a pretend numerical value to indicate the size of the zone in mm, give each one a number 1 – 4 and rank them from most effective to least effective as an antimicrobial.