Aspartate and the enzyme was mixed. After some time, labeled oxaloacetate was added into the mixture. WIll aspartate be observed after oxaloacetate is added?

Organic Chemistry
9th Edition
ISBN:9781305080485
Author:John E. McMurry
Publisher:John E. McMurry
Chapter26: Biomolecules: Amino Acids, Peptides, And Proteins
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Aspartate and the enzyme was mixed. After some time, labeled oxaloacetate was added into the mixture. WIll aspartate be observed after oxaloacetate is added?

Aspartate transaminase is known to display a ping-pong mechanism. This enzyme
catalyzes the following reaction.
II
H2N-CH-C-OH
II
0=C-Ĉ-OH
H2N-CH-C-OH
H2C-C-OH
CH2
CH2
Aspartate
Transaminase
CH2
c=0
CH2
+
CH2
c=0
C=0
c=0
c=0
OH
OH
OH
OH
Aspartate
a-ketoglutarate
Oxaloacetate
Glutamate
This mechanism can be confirmed by isotope exchange experiments.
Transcribed Image Text:Aspartate transaminase is known to display a ping-pong mechanism. This enzyme catalyzes the following reaction. II H2N-CH-C-OH II 0=C-Ĉ-OH H2N-CH-C-OH H2C-C-OH CH2 CH2 Aspartate Transaminase CH2 c=0 CH2 + CH2 c=0 C=0 c=0 c=0 OH OH OH OH Aspartate a-ketoglutarate Oxaloacetate Glutamate This mechanism can be confirmed by isotope exchange experiments.
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