Arginase (an enzyme involved in the hydrolysis of arginine) has an optimum temperature of about 60 °C and has an optimum pH of 9.4. Predict the effect of each of the following on its rate of reaction by answering increase or decrease A. Increasing the temperature from 65°C to 80 °C: B. Changing the pH to from 6.0 to 9.0:

Arginase (an enzyme involved in the hydrolysis of arginine) has an optimum temperature of about 60 °C and has an optimum pH of 9.4. Predict the effect of each of the following on its rate of reaction by answering increase or decrease A. Increasing the temperature from 65°C to 80 °C: B. Changing the pH to from 6.0 to 9.0:

Chemistry for Today: General, Organic, and Biochemistry

9th Edition

ISBN:9781305960060

Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen

Publisher:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen

Chapter20: Enzymes

Section: Chapter Questions

Problem 20.59E

See similar textbooks

Similar questions

When handling or storing solutions of enzymes, the pH is usually kept near 7.0. Explain why.
Most amino acids have isoelectric points between 5.0 and 6.0, but the isoelectric point of lysine is 9.7. Explain why lysine has such a high value for its isoelectric point.
A recent article reported on the development of a new type of opioid drug that binds to a specific protein, but may not have the deleterious side-effects of many opioid drugs currently in use. The new drug is: N- N. The drug binds relatively tightly to the protein at low pH (Kd = 2 nM at pH = 5.5). The drug binds less tightly to the protein at higher pH (Kd = 18 nM at pH 7.4). Consider the drug-binding site in the protein. Explain briefly what aspect of the structure of the binding site could account for this result.
Chymotrypsin is secreted by the pancreas and passed into the intestine. The optimal pH for this enzyme is 7.8. If a patient’s pancreas cannot manufacture chymotrypsin, would it be possible to supply it orally? Justify
b. Aspartate c. Tryptophan d. Alanine 8. Write the products for the following hydrolysis or condensation reactions. a. HgC. HgN NH3 b. H,O 9. How would the following changes affect enzyme activity for an enzyme whose opi conditions are normal body temperature and physiological pH? a. Raising the temperature from 37 °C to 60 °C
Consider the amino acid norvaline: O NH2 OH H C3 a. The pKa of the carboxylic acid group is 2.36. The pKa of the amino group is 9.76. Show how to calculate the isoelectric point, pI of this amino acid. b. Draw the structure of norvaline in a solution of NaOH (aq) c. Draw the structure of norvaline in a solution of H2SO4 (aq)
311. Which is the best binding pocket for phenylalanine? Ho H₂O Ho Ho 99 CH, CH₂ protein c. Ho H;o -CH₂ protein H,O b. H.0 protein HD d. H,0 Ho protein H₂O
7. Amino-Acids: a. Calculate the pl of each of the given amino acids 2.42 7.56 OH o HOT NH3* OH -2.11 4.73 10.34 9.85 -9.97 Compound A Compound B Compound C b. Sketch the major form of compound A from question 7A n a solution at the given pH pH 1 pH 5 thod osvand Valorizą od glasing not alongg +H₂N NH3* duastic OH pH 9 2.31 NH3* pH 12
Complete the table for the ionizable groups of the fictitious amino acid hypothetinine. pka (to TWO decimals) Average charge of group when pH=pK (to ONE decimal) Group Ka alpha-carboxyl 1.38 x 103 alpha-amino 6.02 x 10-10
Draw the structure of alanine in a solution at pH = 0
29.Which amino acid is ideal for the transfer of protons within the catalytic site of enzymes due to the presence of significant amount of both the protonated and deprotonated forms of its side chain at biological pH? A. Lysine B. Asparagine C. Tyrosine D. Cysteine E. Histidine 30. The isoelectric point of alanine is pH = 6.15. It is mixed with proline (pka1 = 2.0; pka2 = 10.6). The mixture is placed in an electric field at pH 6.15 and then subjected to isoelectric focusing. Which statement is true? A. The two amino acids will be separated B. The two amino acids will not be separated C. Neither amino acids will move in the electric field D. Both amino acids will move from the origin and separated
Quesrion: Enzyme X exhibits maximum activity at pH = 10.5. X shows a fairly sharp decrease in its activity when the pH goes much lower than 9.5. One likely interpretation of this pH activity is that: a. A His residue on the enzyme is involved in the reaction b. a Glu residue on the enzyme is involved in the reaction c. a Tyr residue on the enzyme is involved in the reaction d. the enzyme uses NADH has a cofactor e. the enzyme uses coenzyme A has a cofactor Please explain why, thanks!