Analytical wavelength Analytical wavelength for yellow dye (nm) Calibration curve Solution 1 2 3 4 5 Dye concentration (µM) 28.10 14.00 Unknown solution 7.02 3.51 1.76 Molar absorptivity (μM-¹cm-¹) Absorbance Concentration of unknown yellow dye (μm) 427 Absorbance 0.713 0.358 0.189 0.084 0.057 0.0251 0.157

Analytical wavelength Analytical wavelength for yellow dye (nm) Calibration curve Solution 1 2 3 4 5 Dye concentration (µM) 28.10 14.00 Unknown solution 7.02 3.51 1.76 Molar absorptivity (μM-¹cm-¹) Absorbance Concentration of unknown yellow dye (μm) 427 Absorbance 0.713 0.358 0.189 0.084 0.057 0.0251 0.157

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry

Section: Chapter Questions

Problem 9.11QAP

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A student prepared several solutions of Molecule Bright, each at a different concentration. The absorbance of each solution was measured at 405 nm with a path length of 0.730 cm. The student then plotted the absorbance of each sample vs. its concentration (in mM), and found a best-fit line of y = 3.726x + 0.828. Calculate the molar absorptivity of the Molecule Bright (in M-1 cm-1).
Suppose you measure the absorbance of a yellow dye solution in a 1.00 cm cuvette (b=1.00 cm). The absorbance of the solution at 427 nm is 0.84 . If the molar extinction coefficient of yellow dye at 427 nm is 27400 M–1cm–1, what is the concentration of the solution in M?
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