Amount of B-Galactosidase (% of mutant strain Addition of Lactose in the presence of lactose) Mutant No 100 Mutant Yes 100 Merozygote No 100 Merozygote Yes 200 Explain the nature of the defect in the mutant strain.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter26: Synthesis And Degradation Of Nucleotides
Section: Chapter Questions
Problem 10P
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Suppose that you have isolated a mutant strain of E. coli in
which the lac operon is constitutively expressed. To understand
the nature of this defect, you create a merozygote in which the
mutant strain contains an F′ factor with a normal lac operon
and a normal lacI gene. You then compare the mutant strain and
the merozygote with regard to their β-galactosidase activities in
the presence and absence of lactose. You obtain the following
results:

Amount of B-Galactosidase
(% of mutant strain
Addition of Lactose in the presence of lactose)
Mutant
No
100
Mutant
Yes
100
Merozygote
No
100
Merozygote
Yes
200
Explain the nature of the defect in the mutant strain.
Transcribed Image Text:Amount of B-Galactosidase (% of mutant strain Addition of Lactose in the presence of lactose) Mutant No 100 Mutant Yes 100 Merozygote No 100 Merozygote Yes 200 Explain the nature of the defect in the mutant strain.
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