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- The aromatic side chain of Trp residues in proteins and peptides can be chemically modified by two photochemical reactions using ultraviolet light (hv) at 20°C (Figure 1). hv CCI3CO₂H (10 mm), Buffer A (pH 8.0) O HO HN HN- O A. Acidic NH ww O B. Basic O c. Hydrophobic O D. Polar neutral OB. Indole NH ww Figure 1 Photochemical reactions of Trp side chain with CHCl3 and CCI₂CO₂H O A. Imidazole Each substitution on Trp replaces one hydrogen atom attached to a carbon somewhere on the indole ring. Photochemical modification percentages of various Trp residues in carbonic anhydrase at 20°C are shown in Table 1. Table 1 Photochemically Modified in Carbonic Anhydrase Reactant Buffer W4 W15 W96 W122 W190 W207 W243 51% 0% 44% A 37% 0% 0% CHCI 3 0% CHCI₂ B 0% 0% 0% 0% 0% 0% 55% CCI,CO₂H A 72% 66% 0% 100% 100% 0% 75% O c. Pyrimidine OD. Pyrrole hv W15 and W96, which are in the interior of carbonic anhydrase, are 21% and 29% modified respectively by 10 mM CHCl3 in Buffer A at 75°C, a temperature…Provide the following information about L-dopa. a. Label all sp hybridized C atoms. b. Label all H atoms that bear a partial positive charge (8"). c. Draw another resonance structure.The polymer chains of glycosaminoglycans are widelyspread apart and bind large amounts of water.a. What two functional groups of the polymer make thisbinding of water possible?b. What type of bonding is involved?
- Two peptide sequences are shown below.Peptide A: N-term – GDL – C-termPeptide B: N-term – GTL – C-terma. Draw each peptide at physiological pH. Circle each ionizable functional group in your drawing.b. Briefly explain why changing aspartate to threonine changes the number of ionizable functionalgroups in the peptide chain.c. Briefly explain why the functional group found in aspartate’s side chain is ionizable but the functionalgroup found in threonine’s side chain is not.1. What is the possible identity of the AA? a. Aspartic acid b. Lysine c. Alanine d. Proline 2. What is the isoelectric point of AA? 3. What is the pKa responding to the dissociation of the alpha-carboxylic group?Which of the following shows the reaction by which valine and leucine form a peptide bond H. H. H O -COO H2N-C-C-NH- C- C-OH + HO CH2 CH,SH ČH3 А. C. CH(CH)2 H. HN-C-C-NH-C- C-OH H,N-CH-C- + H,O HC-C-H CH2 CH2 H CH3 CH H,C-C-H CH(CH3)2 CH В. D.
- Based on the structure below: 1. Based on the chemistry, is the drug phototoxic? (Yes or No) 2. Is the drug is acid-stable? (Yes or No) 3. Which of the following is TRUE about the structure shown? A. The drug is doxycycline. B. The drug is resistant against dehydration. C. The drug forms into a lactone when dehydrated. The drug is bactericidal. D. The drug is phototoxic.Which of the following statements are TRUE? Multiple answers:Multiple answers are accepted for this question PLEASE SHOW WORK FOR EACH PART AND EXPLAIN WHY EACH ANSWER CHOICE IS WRONG OR RIGHT a. The tripeptide His-Lys-Glu has a net charge of -2 at pH 8.0. b. The tripeptide Asp-Asp-Asp has a net charge of -3 at pH 7.0. c. The tetrapeptide His-His-His-His has a net charge of -1 at pH 7.0. d. There are 6 possible sequences for a tripeptide containing Arg - His - and Pro. e. Certain bacteria synthesize cyclic tetrapeptides. The net charge at pH 7.0 of a cyclic tetrapeptide consisting of two Pro and two Tyr is 0.1. a. draw the L- & R- stereoisomer of the amino acid T using a fischer projection or a newman projection. b. draw the tripeptide LEM in the protonation state you would expect at physiological pH. show the position of phi and psi angles on the E residue
- Draw the structure of phosphatidylcholine at pH 7 with the moiety on R₁ as 14:0 (C14) and R₂ as 12:2cis,cis-A6,9. Put an X on the bond cleaved by Phospholipase C.What would be the molecular formula for a linear oligomer made by linking ten glycine molecules together by condensation synthesis? * The molecular formula for glycine is C2H5O2N. A. C20H32011N10 B. C20H50020 N10 C. C20H68O29 N10 D. C20H40010N10PREDICT Do any of the amino acid side groups shown below have the potential to form an ionic bond with any of the other side groups shown? If so, which pair(s) could form such an association? (a)CH3 (b)CH2 COO (c)CH2 CH2 NH3+ (d)CH2 CH2 COO (e)CH2 OH