a. The substrate makes aDUT less stable and $DUT more stable

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter18: Glycolysis
Section: Chapter Questions
Problem 25P: Using the ActiveModel for phosphofructokinase (Trypanosoma), describe the difference between the...
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3. MUTPase from ASFV (aDUT) and from swine (listed as sDUT) were studied in the absence and present of the
DUTP substrate. Using the melting temperature data provided below, how does adding the substrate affect
enzyme stability? Explain your choice in 25 words or less.
TemperatureL°CT
protein
Tm by thermal denaturation (°C)
aDUT
83.1 ± 0.2
SDUT
61.8 + 0.2
aDUT-DUTP-Mg
SDUT-dUTP-Mg
84.5 + 0.1
62.7 + 0.2
a. The substrate makes aDUT less stable and SDUT more stable
b. The substrate makes aDUT more stable and $DUT less stable
c. The substrate makes both ADUT and SDUT less stable
d.
The substrate makes both aDUT and SDUT more stable
sh (United States)
D. Focus
rch
7:15
80°F
ENG
7/10/2
DELL
F3
F4
Transcribed Image Text:3. MUTPase from ASFV (aDUT) and from swine (listed as sDUT) were studied in the absence and present of the DUTP substrate. Using the melting temperature data provided below, how does adding the substrate affect enzyme stability? Explain your choice in 25 words or less. TemperatureL°CT protein Tm by thermal denaturation (°C) aDUT 83.1 ± 0.2 SDUT 61.8 + 0.2 aDUT-DUTP-Mg SDUT-dUTP-Mg 84.5 + 0.1 62.7 + 0.2 a. The substrate makes aDUT less stable and SDUT more stable b. The substrate makes aDUT more stable and $DUT less stable c. The substrate makes both ADUT and SDUT less stable d. The substrate makes both aDUT and SDUT more stable sh (United States) D. Focus rch 7:15 80°F ENG 7/10/2 DELL F3 F4
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