A solute with a retention time of 400.0 s has a width at half-height of 8.0 s on a column 12.2 m long. Find the number of plates and the plate height.
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A solute with a retention time of 400.0 s has a width at half-height of 8.0 s on a column 12.2 m long. Find the number of plates and the plate height.
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- A solute with a retention time of 407 seconds has a width at the base of 13.0 seconds on a column 12.2 meters long. Find the number of plates and plate height. The half-width of the same peak is 7.6 seconds. Find the plate height.From the data calculate: a) retention factor of each solute b) selectivity factor c) how many plates are needed to provide a resolution of 1.5? d)how long a column is needed if the plate height of the packing is 1.5x10^-3cm? e)if the flow rate is 6.75 cm/min how long will it take to elite the two species?4.columns with one million theoretical plates operating by slip flow. The figure shows the gradient separation of two forms of fluorescently labeled bovine serum albumin separated on a)50 x 2.1 mm UHPLC column of 1.7 µm C4 silica particles and b)10 mm x 75µm colloidal crystal column of 470 nm C4 silica nanoparticles. What are the main differences in the chromatogram? What is the reason for the difference? Why does the colloidal crystal column have such a high number of theoretical plates (N)? How is the problem of high pressures with very small particles overcome?
- A solute has a retention time of 5.4 min with a width at half height of 0.41 min on a column 12.2 m long. Find the number of plates. Please round-off your answer to a whole number (no decimal place)16) Which of the following(s) is/are correct? I- Selectivity factor is the amount of time a solute spends in the stationary phase relative to the time it spends in mobile phase II- The retention time for an analyte is the time interval between its injection onto a column and its appearance at the detector at the other end of the column. III- Distribution constant is the ratio of the total solute concentration in the stationary phase to that in the mobile phase. A) I, II and III B) Only II C) I and III D) II and III E) Only III for GC27 How is the variation in sample composition in a powder random mix influenced by the proportion of active ingredient particles and by the total number of particles in the sample?
- Consider a 22.0 cm long column with a plate height of 0.0125 mm, a flow rate of 1.0 mL/min, and a Vm =1.2 mL (peak shapes are all symmetric). What is the solute retention time, retention volume, and peak width at half height when k’ = 7.2?A chromatography column has a stationary phase volume that is 0.130 times the volume of the mobile phase. Find the retention factor, k, for a solute with each of the given distribution constants, Kp. Кр 3D 2.68 k = Kp = 19.1 k =In a reverse-phase separation, a solute was found to have a retention time of 19.6 min, while an unretained peak had a retention time of 1.45 min when the mobile phase was 20/80 v/v% methanol/water. (b) Calculate the k’ for the solute. (c) Calculate the new k’ if the mobile phase is changed to 60/40 methanol/water assuming identical void volume. (d) Calculate a solvent composition that would bring k’ from initial value to a value of 1.20 using tetrahydrofuran/water mobile phase rather than 20/80 v/v % methanol/water.
- What is the minimum distribution constant that permits removal of 99% of a solute from 50.0 mL of water with two 25.0-mL extractions with toluene? five 10.0-mL extractions with toluene?From a 10-mL sample, a 1-mL aliquot was taken and diluted to 100mL. From this, a 5-mL aliquot was taken and diluted to 20mL.The final 20mL was found to have a concentration of 0.004M analyte X.What is the concentration of analyte X in the 10-mL sample?Effect of Particle Size on the Rate of Dissolving Time required for all NaCl to dissolve Sample Results after 2 minutes shaking Do you think the size of the crystal makes a difference in how fast the salt dissolves? Explain why the differences you observed and why they happened. Effect of Temperature on the Rate of Dissolving Time required for all NaCl to dissolve Sample Observations Do you think the temperature of the water makes a difference in the rate at which the NaCl dissolves? Explain why the differences you observed and why they happened. Page 3 of 6