Homework Help is Here – Start Your Trial Now!
Science
Chemistry
(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table 1 was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Tube Number Standard (mM)/Test sample A 1 0 2 0.025 3 0.05 4 0.075 0.1 (i) (ii) 5 Absorbance @575nm 6 7 8 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Test sample A (undiluted) Test sample A (2-fold dilution) Test sample A (5-fold dilution) Using the data in Table 1 estimate the amino acid concentration of test sample A. Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimernal data. (iii) Describe the principle of the Ninhydrin method for quantitation of amino acids.

(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table 1 was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Tube Number Standard (mM)/Test sample A 1 0 2 0.025 3 0.05 4 0.075 0.1 (i) (ii) 5 Absorbance @575nm 6 7 8 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Test sample A (undiluted) Test sample A (2-fold dilution) Test sample A (5-fold dilution) Using the data in Table 1 estimate the amino acid concentration of test sample A. Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimernal data. (iii) Describe the principle of the Ninhydrin method for quantitation of amino acids.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
See similar textbooks
icon
Related questions
Question

help please  

(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table 1 was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Tube Number Standard (mM)/ Test sample A 0 0.025 0.05 0.075 0.1 Test sample A (undiluted) e (1) (ii) 1 2 3 4 5 6 7 8 Test sample A (2-fold dilution) Test sample A (5-fold dilution) Absorbance @575nm 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Using the data in Table 1 estimate the amino acid concentration of test sample A. Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimental data. Describe the principle of the Ninhydrin method for quantitation of amino acids.
Transcribed Image Text:(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table 1 was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Tube Number Standard (mM)/ Test sample A 0 0.025 0.05 0.075 0.1 Test sample A (undiluted) e (1) (ii) 1 2 3 4 5 6 7 8 Test sample A (2-fold dilution) Test sample A (5-fold dilution) Absorbance @575nm 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Using the data in Table 1 estimate the amino acid concentration of test sample A. Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimental data. Describe the principle of the Ninhydrin method for quantitation of amino acids.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 5 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Mass Spectrometry
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
SEE MORE TEXTBOOKS