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- 5. Using the diagram answer the questions below: Increasing time Sample applied p to top of column Column fow (a) eluent eluent eluent eluent (A) Draw a sketch of the expected chromatrogram (peaks, broadening, relative elution times, etc.) (B) Explain the term "analyte dilution" using the diagram as a reference.cont irmation True or false: The purpose of the internal standard is to make quantitation of the analyte insensitive to injection errorsDerivatiztion of sample is carried out to: a) Improve analyte peak shape analyte resolution b) Increase the detector response c) Improve d) All the above
- What is the role of specific visualizing agents in a Thin layer Chromatography? Please shortly answer at your own words. Answer should be to the point (4-5 lines).(b) For column 1(i) What are the unadjusted retention times for components A and B?Ooldtion Table 1. Measurements for standardization of NaOH Trial 1 Trial 2 Trial 3 1] Tared mass of KHC3H4O4 (9) 0.374 0.325 0.357 12] Burette reading of NaOH, initial (mL) 4.59 3.61 4.03 [3] Burette reading of NaOH, final (mL) 22.03 18.77 21.17 Part B. Molar Concentration of an Acid Solution Enter the number corresponding to the type of acid you have 1. HA 3 2. H2A 3. H3A Enter the unknown number. 21 Table 2. Measurements for titration of unknown acid Trial 1 Trial 2 Trial 3 [1] Volume of acid (mL) 25.0 25.0 25.0 [2] Burette reading of NaOH, initial (mL) 2.13 2.48 3.68 [3] Burette reading of NaOH, final (mL) 24.10 24.52 25.56 Part C. Percentage by Mass of an Unknown Solid Enter the number corresponding to the type of acid you have 1. HA 3 2. H2A 3. H3A Enter the unknown number. 30 Molar mass of the unknown acid 192.14 Table 3. Measurements for titriation of solid Trial 1 Trial 2 Trial 3 [1] Mass of sample (g) 26.334 29.157 27.111 [2] Burette reading of NaOH, initial (mL) 3.21 1.48…
- A sample of explosives residue is dissolved in an appropriate solvent and is injected into HPLC. The column contains a non- polar stationary phase. The sample revealed three chemical compounds that are commonly present in homemade explosives. ТAТP DADP HMTD 1 2 3 4 5 6 7 8 Retention time (minutes) a) determine the retention time of TATP. b) which one elute first :DADP,TATP or HMTD? c) State and explain which compound is most polar. d) If you are investigating this terrorist attacks, state one way in which the information in the chromatogram would be useful.. 100 ml boiled cooled and filtered water sample takes 9.6 ml of M/50 EDTA in titration. The Permanent hardness of the water sample in terms of ppm of CaCO3 equivalent isThis method is to be used when measuring the volume of a solution containing sufficient reagent to react completely with the analyte. Volumetric O Spectroscopic Gravimetric Electroanalytical
- what can be an analyte for using internal standard? sample: vegetable oil containing ethyl oleate, IS: Ethyl oleate. what should be analyte here (what mixture composions) to use for internal standard method and calibration?of the analyte increases, As the temperature of a gas chromatograplhy column increases, the the retention time , and the peak shape Select one: O a. temperature; increases; sharpens O b. vapor pressure; decreases; sharpens O c. equilibrium; increases; broadens O d. vapor pressure; increases; sharpens O e. solubility; decreases; sharpensDirections: (1) Determine the total volume of the standards by using a formula in the appropriate boxes (your instructor will help you with this). (2) Determine the concentration of Cu2+ in each standard, also using a formula in the appropriate cells. (3) Plot the absorbance as a function of Cu2+ concentration. Determine the equation for the best-fit line. 1.0 M ethylenediamine (mL) concentration Cu2+ absorbance (AU) at 550 nm 0.01 M CuSO4 5H2O H2O total volume stock Cu2+ standard (mL) (mL) (mL) (units) concentration (M) 1 0.50 0.00 9.50 0.025 0.01 2 0.50 0.50 9.00 0.031 0.01 3 0.50 1.00 8.50 0.048 0.01 4 0.50 2.00 7.50 0.153 0.01 5 0.50 3.00 6.50 0.223 0.01 0.50 4.00 5.50 0.307 0.01 7 0.50 5.00 4.50 0.384 0.01