A buffer used in an experiment is 20 mM HEPES with a pH of 7.7. 25mM KCI, 5mM MgCl2 buffer. Explain how you would prepare a 300 mL, 100 mM stock solution of this HEPES buffer? Show all calculations. For KOH use (1M) to adjust pH and this is occurring at 25°C
A buffer used in an experiment is 20 mM HEPES with a pH of 7.7. 25mM KCI, 5mM MgCl2 buffer. Explain how you would prepare a 300 mL, 100 mM stock solution of this HEPES buffer? Show all calculations. For KOH use (1M) to adjust pH and this is occurring at 25°C
Chemistry: Principles and Reactions
8th Edition
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:William L. Masterton, Cecile N. Hurley
Chapter14: Equilibria In Acid-base Solutions
Section: Chapter Questions
Problem 12QAP: A buffer is prepared by dissolving 0.062 mol of sodium fluoride in 127 mL of 0.0399 M hydrofluoric...
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