9. For each of the following experimental goals, is PCR or gene cloning preferable and why? a. Isolate the same gene from 20 individuals. b. Isolate 100 genes from the same individual c. Isolate a mouse gene when you have a rat-gene fragment.
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- 1. What is the purpose of restriction enzyme? 2. What is the purpose of the probe in DNA finger printing. 3. What is/are the sample that can be used for DNA finger printing? 4. Who is the culprit base on our interactive laboratory experiment?1. What happens in the denaturing step of PCR? A. What happens during the annealing step of PCR? B. What happens during the extension step of PCR? C. What temperatures is associated with each PCR step? D. Why is it necessary to have a primer on each side of the DNA segment to be amplified? (Hint: draw it out to see what happens with only one primer). E. How did the Taq DNA polymerase acquire its name? F. Why are there nucleotides (A, T, G, C) in the master mix? What are the other components of the master mix and what are their functions?For each of the following experimental goals, is PCR orgene cloning preferable and why?a. Isolate the same gene from 20 individuals.b. Isolate 100 genes from the same individual.c. Isolate a mouse gene when you have a rat genefragment.
- About transcriptomics and genomics, answer: 4. Can we replicate RNA? How can we use PCR and Sanger sequencing to study the ranscriptome? a. b. How will the genome of two different cells of the same animal compare? How will the transcriptome oftwo different cells of the animal compare? c.What is the proper order of the following steps in a gene-cloningexperiment involving vectors?1. Add DNA ligase.2. Incubate the chromosomal DNA and the vector DNA with arestriction enzyme.3. Introduce the DNA into living cells.4. Mix the chromosomal DNA and vector DNA together.a. 1, 2, 3, 4b. 2, 3, 1, 4c. 2, 4, 1, 3d. 1, 2, 4, 3a. What determines the size (length) of the primary PCR product? b. What might a successful gel check of a PCR reaction look like?
- Q. How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and reverse primers that bind to the same exon. B. Run a control lane where only RT was performed and not PCR. C. Run a control lane where mRNA has been amplified using PCR. D. Use forward and reverse primers that span the junction of 2 separate exons.1. Introduction: Define the following concepts and processes 1) List material used in a PCR reaction 2) List steps of PCR reaction and temperatures for each step1. Explain what primers are and what purpose they serve in a PCR reaction. Explain the main steps and temperatures of one PCR cycle. 2. Explain the purposes of the following components of gel electrophoresis: agarose, loading buffer, DNA ladder/marker, electrode buffer, electrodes. 3. Explain the expected results of the Alu sequence at PV92 that we tested, in terms of numbers of bands and their sizes. A labeled drawing would suffice.
- What is the most challenging issue facing genome sequencing? a. the inability to develop fast and accurate sequencing techniques b. the ethics of using information from genomes at the individual level c. the availability and stability of DNA d. all of the aboveWhich of the following steps is NOT involved in gene cloning? Lütfen birini seçin: O a. Transferring recombinant vector into the host cell O b. Insertion of a gene into a vector O c. Selection of cells with recombinant vectors O d. Removing nucleus from donor cell O e. Isolation of a gene to be clonedVII. Match the technique described to the application given. DNA amplification b. РCR c. gel electrophoresis d. STR profiling dideoxy sequencing f. automated DNA sequencing g. site directed mutagenesis gene therapy h. 97. Nucleotides missing 3'-OH group makes small pieces that can be analyzed for identification. 98. DNA amplification without bacteria that includes cycling temperature changes to produce multiple copies of small pieces. 99. Identification of DNA by a series of peaks made from fluorescing based on fragment length. VIII. Match each item to the correct statement below. f. negative feedback system g. excretory system h. autonomic system i. positive feedback system j. parasympathetic nervous system homeostasis b. somatic system a. C. nervous system d. sympathetic nervous system endocrine system е. 100. way in which the body works to reverse a change and bring about normalcy 101. the tendency of the body to maintain a constant internal environment IX. Match each item to the…
