9) How many peptide fragments would you expect to generate from reacting the following amino acid sequence with the hydrolytic enzyme trypsin, followed by a reaction with cyanogens bromide? LPETYADFHGWQPLMNCVSDEJHGAQJIYTL

9) How many peptide fragments would you expect to generate from reacting the following amino acid sequence with the hydrolytic enzyme trypsin, followed by a reaction with cyanogens bromide? LPETYADFHGWQPLMNCVSDEJHGAQJIYTL

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section10.7: Defects In Transport Proteins: Hemoglobin

Problem 1EG

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9) How many peptide fragments would you expect to generate from reacting the following amino acid
sequence with the hydrolytic enzyme trypsin, followed by a reaction with cyanogens bromide?
LPETYADFHGWQPLMNCVSDEJHGAQIIYTL

8) One variant of hemoglobin (Variant X) has a pso = 30 torr; another variant of hemoglobin has a pso =
40 torr (Variant Y). Circle the variant with the greatest affinity for dioxygen.
Variant X
Variant Y

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The figure shows interaction of various amino acids (Phe, Arg, and Asn) present in Hb with 2,3-DPG. The side chains of these amino acids and 2,3-DPG interactions are circled in red. HO Но Phe HN Asn H2N NH Arg You identify a mutant version of the globin chain where the Arg in circle 2 is replaced with aspartic acid in a patient. Would the Hb protein in this patient be able to transport oxygen even in the presence of 2,3-DPG? ASPARTIC ACID (asp) No, because the mutant Hb version will bind 2,3 DPG, and not oxygen Yes, because the mutant Hb version will bind 2,3 DPG, and not oxygen Yes, because the mutant Hb version will bind oxygen and not 2,3 DPG
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