83. A plasmid is cut with the restriction enzyme BamH1 giving fragments of 3000 and 1000 bp as identified by gel electrophoresis and ethidium bromide staining. In a seperate restriction digest the enzyme EcoR1 gives fragments of 1000 and 1500 bp that are apparent on the agarose gel. What is the most likely size of the plasmid in bp? Explain why it's 4000
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
83. A plasmid is cut with the restriction enzyme BamH1 giving fragments of 3000 and 1000 bp as identified
by gel electrophoresis and ethidium bromide staining. In a seperate restriction digest the enzyme EcoR1
gives fragments of 1000 and 1500 bp that are apparent on the agarose gel. What is the most likely size of
the plasmid in bp?
Explain why it's 4000
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