281Customized Subject Test50. During an experiment, equal aliquots of Escherichia coli and Staphylococcus aureus are separately sonicated at the same level to mechanically disrupt bacterial cells.E. coli cells are disrupted, but there is minimal disruption of S. aureus cells. Which of the following bacterial structures best explains this difference?A) Cytoplasmic membraneB) Outer membraneC) Peptidoglycan layerOD) Phospholipid bilayerOE) Polysaccharide capsule

Introduction:- The peptidoglycan layer is a component of the bacterial cell wall that provides…

Answered: 50. During an experiment, equal…

Phlebotomy Essentials

6th Edition

ISBN:9781451194524

Author:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Publisher:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Chapter1: Phlebotomy: Past And Present And The Healthcare Setting

Section: Chapter Questions

Problem 1SRQ

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50. During an experiment, equal aliquots of Escherichia coli and Staphylococcus aureus are separately sonicated at the same level to mechanically disrupt bacterial cells. E. coli cells are disrupted, but there is minimal disruption of S. aureus cells. Which of the following bacterial structures best explains this difference? OA) Cytoplasmic membrane OB) Outer membrane OC) Peptidoglycan layer OD) Phospholipid bilayer OE) Polysaccharide capsule
All questions a) Name structures P, Q and R. b) Name substance S . c) What is the enzyme encoded by gene I. Give its function. d) What will happen if substance S is absent in the medium?
The correct order of steps when purifying a protein are: Group of answer choices 1)Growing bacterial culture for fusion protein; 2)Harvesting IPTG-induced cultures; 3)Lysing bacterial cell; 4)Removing insoluble debris; 5)Using Affinity Chromatography to purify protein. 1)Growing bacterial culture for fusion protein; 2) Breaking open bacterial cells; 3) Removing insoluble debris: 4) Harvesting IPTG-induced cultures; 5)Using Affinity Chromatography to purify protein 1) Growing bacterial culture for fusion protein; 2) Lysing bacterial cells; 3) Harvesting IPTG-induced cultures; 4) Removing insoluble debris: 5)Purifying protein by affinity chromatography 1)Growing bacterial culture for fusion protein; 2) Removing insoluble debris; 3) Harvesting IPTG-induced cultures; 4)Breaking open bacterial cells; 5)Purifying protein by affinity chromatography
Briefly explain that what is the important to use immobilization techniques in biosensor?..(explain under5 points)
Following on from the last question, what assays could you do to find out if your mitochondria are pure? Select all that apply A) Assay succinate dehydrogenase to confirm mitochondria are present B) Assay alkaline phosphatase to check for contamination with Lysosomes C) Measure protein using the Bradford assay D) Assay acid phosphatase to check for contamination with Lysosomes E) Assay lactate dehydrogenase to confirm mitochondria are present
Regarding the use of animal cells in culture, it is correct to state that: * (a) Low cost culture media composed of mineral salts and amino acids are used (b) Cells must be kept in semi-solid media, allowing them to come into contact with the atmosphere rich in carbon dioxide. (c) Greenhouses with controlled temperature and atmosphere composed predominantly of carbon monoxide are used (d) On an industrial scale, culture media supplemented with serum of animal origin are used, which makes them media of indefinite composition (e) Culture media must not be sterilized by autoclaving, preferably filtration and/or ionizing radiation methods are used
52. During the development of a new vaccine, an investigator generates a mutant strain of bacteria that does not adhere to mucosal epithelia, Mutations in which of the following bacterial components or structures would most likely prevent bacterial adhesion? htba OA) Capsules OB) Flagella OC) Lipopolysaccharide OD) Lipoteichoic acid OE) Pili OF) Porins OG) Siderophores OH) Spores in 1thou (moon) Pili
Aeroderma pigmentosum patients have a 10,000-fold increase in the risk of developing Skin cancer. These patients have to avoid exposure to UV light. Please explain why. To answer the question please: I) name the process that is affected and provide a scheme; 2) indicate template, substrates, sources of energy that are involved in the process; 3) propose the consequences of the process.
4) If 20% of a culture of human cells have a DNA content somewhere between 1&2xs (S-phase= 8 hours) and 1X amount of DNA in non-dividing cells, what is the generation time? A) 25 Hrs B) 30 hrs C) 0.025 hrs D) 40 hrs E) None of these
1) The inactivation of Staphylococcus aureus was studied in pomegranate juice heated at 75°- 95°C. The slopes for the inactivation of this bacteria were calculated from the linear lines in semi-log graphical paper. The slope values were presented in Table 1. a) Determine the temperature change needed to inactivate this bacteria by 90%. b) Temperature change to inactivate Bacillus cereus in pomegranate juice by 90% at the same temperature range was 29°F. At 75°-95°C temperature range, which bacteria will be more affected by the temperature changes? Commend on your answer in one sentence Table 1- Slope values for the inactivation of Staphylococcus aureus in pomegranate juice heated at various temperature Temp. (°C) 75 80 85 95 -Slope x 10² (min-¹) 2.222 3.704 5.000 13.158
In the experiment by Bernard Davis, bacterial F+cells and F- cells were growing while separated by a filter. Filter pores allowed the passage of the liquid medium but not the bacteria cells. As a result: 1) prototype colonies grew well on minimal medium 2) F+ cells were converted to F- cells despite the physical separation 3)F- cells were converted F+ cells despite the physical separation 4)F+ cells were not converted to F- cells because of the physical separation 5) F- cells were not converted to F+ cells because of the physical separation 6)there was no growth of prototypes on minimal medium
What is the difference and compare the light source and path of light of a immunofluorescence microscope from a compound light microscope?

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