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- Refer to the description and table below to answer the three question that follow: Dissolved oxygen levels from 3 bottles of algae were recorded. The DO level of bottle #1 was recorded immediately after the algae was collected, the DO level of bottle #2 was recorded after sitting for 20 hours wrapped in tin-foil, and the DO level of bottle #3 was recorded after sitting for 20 hours under a lamp. A heat sink was placed over all of the bottles to ensure that temperature did not have an effect on the results. The following data was collected: Bottle Number D.O. Level 8 #1 #2 1 #3 6 4. How many units of oxygen were used for cell respiration by the algae? a. 7 b. 2 c. 5 d. -2Answer the question briefly and concisely. The Mannitol salt agar (MSA) only allows the growth of halophiles (salt-loving microbes);nonhalophiles will not grow. Among the halophiles, mannitol fermenters will produce acid that turnsthe pH indicator yellow; mannitol nonfermenters leave the medium red. Onto MSA you inoculate ahalophilic mannitol fermenter and a halophilic mannitol nonfermenter. In this case, the medium isacting as (a) __________ medium(s).A. SelectiveB. DifferentialC. Selective and differentialD. EnrichedExpalin in detail the one use and one potential environmental risk of sludge usage.
- Briefly explain the term cardinal temperatures and apply it to the terms psychrotrophic and mesophilic microorganism.Which microhabitat supports growth of halophilic microorganisms? Check all that apply. * Great Salt Lake bagoong (fish paste) Dead Sea fruit jam & jellystate 2 advantages and two disadvantages of using ; radioisotopic and stable isotopic methods in the study of microbial ecology.
- Using the knowledge on biological wastewater treatment , what conclusion would you derive from the data given at the end? Parameter Unit TAI-I-11 1007 TAI-E-11 1007 AIR-I-11 1007 AIR-E-11 1007 CB-I-11 1007 CB-E-11 1007 COL-I-11 1006 COL-E-11 1006 MAC-I-11 1006 MAC-E-11 1006 SS mg/L 204 28 40 16 36 26 196 132 132 122 COD 503 83 256 149 156 63 437 234 459 269 BOD5 266 28.0 102 52.8 72 14.6 232 89.2 168 55.2 NH3-N 23.6 14.8 30.7 18.3 14.2 0.5 20.1 4.0 21.3 18.5 TP 6.4 3.2 10.9 7.6 3.0 1.9 4.2 2.4 4.6 4.5 TN 43 29 45 30 19 10 29 15 27 25 Fecal Coliforms (FC) MPN/ 100 mL 1.4x104 8x102 2x103 1.7x103 3.4x104 8 4x103 23 1.7x104 9x103 Color Unit Pt/Co 191 45 283 187 154 26 108 37 138 57 pH 7.34 7.59 7.62 7.64 7.15 7.79 7.03 7.46 7.35 7.32 Note…Relate the terms presented in the first column with the information in the second column; i. Temperatureii. face speediii. Relative humidityiv. Transport and storage timev. Exposure time and concentration of pollutants (__) May influence the integrity of the sample;(__) Influence the amount of sample collected(__) Defines the choice between a sampler containing hydrophilic adsorbents or one with hydrophobic adsorbents.(__) Influences the effective length of the diffusion path.(__)Variation in sampling due to its influence on the diffusion coefficientMicroorganism growth in complex natural environments such as soils and waters can be utilized to carry out environmental management processes. Give one term for the process you would use to cleanse a marine oil spill and discuss at least 3 types thereof. Outline factors that would facilitate the success of the process you mentioned on 1. Discuss the use of indicator organisms in testing the quality of drinking water.
- TOPIC : MICROBIAL GROWTH Classify the bacteria that is most likely involved in each situation provided according to their classification (as to microbial growth requirements). CHOICES: A. Obligate aerobe ,B. Obligate anaerobe, C. Microaerophile, D. Aerotolerant anaerobe, E. Mesophile, F. Thermophiles 1.The bacteria under study thrives at the alveolar ducts in the lungs causing respiratory disease. 2.In the experiment, the agar plate containing an axenic culture was originally incubated inside a candle jar for one day and then transferred to an oxygen-rich incubator for another day. Findings: the microbial growth persisted and stayed the same regardless of the specific type of incubation employed. 3.A microorganism that can survive pickling and refrigeration was discovered when a tightly-sealed jar of pickles grew several colonies despite following the correct recipe, tight closure seal, and proper refrigeration. 4.The spoiled bottle of wine that was stored at standard room temperature…Write an abstract of the article below in Methods (around 3 sentences): METHODSTo accomplish this, three kinds of inocula - Pond bottom soil 1, Pond bottom soil 2 and Horse dung - wereobtained from two different ponds and outdoor loafing sheds near Keller, Texas. Waste samples, including food,paper and yard waste, were collected from a house. Three laboratory scale reactors were prepared with selectedhousehold waste and inoculum with the required amount of water and operated at room temperature. Duplicateruns were conducted for the first set of experiments. The pH level of reactors was controlled by adding sodiumhydroxide.The activated sludge process was developed from empirical knowledge and is now widely used for wastewater treatment. From a microbial point of view, i. Discuss how the ecology of different sectors of the process can be defined. ii. Design an experiment to understand how an efficient process differs from one with problems, such as sludge bulking.