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- Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…at Arrange Window Help 4) 57% D Tue 4 May Tools Slide Show 2 ARMS PCR nations Slide Show Review View A A =、=<|EE|三v| v v A Sha v 20 Quick Styles D Sha Text Arrange Convert to SmartArt Picture Shapes ab x x. AV v Aa v D. Av A、申。 Box Briefly outline how MLPA probe-sets can simultaneously detect multiple targets using a single set of PCR primers. E Notes Comments MAY 4 Pr w MacBook Air24. Arrange the correct sequence in southern blotting. I. DNA extracted from white cells and are cleaved. II. hybridization with p-labeled probe. III. Agarose gel electrophoresis. IV. Blotting technique V. Expose of x-ray film. O A. I, III, II, V, IV ) B.I, II, III, IV, V. OC.I, IV, III, V, II D. I, III, IV, II, V. Clear selection O O O
- 2. The following data were generated after a set of bovine serum albumin standards were measured via the BCA assay. Table 1. Absorbance measurements at 562 nm for BSA standards measured in triplicate in a BCA Assay. Standards A B C D E F G H I Conc (ug/ml) 2000 1500 1000 750 500 250 125 25 0 Replicate 1 Abs @ 562 nm 2.381 2.039 1.505 1.143 0.819 0.454 0.245 0.056 0 Replicate 2 Abs @ 562 nm 2.456 2.052 1.489 1.156 0.823 0.46 0.272 0.049 0 Replicate 3 Abs @ 562 nm 2.43 2.05 1.47 1.17 0.81 0.48 0.255 0.053 0.00 Use Excel or your graphing program of choice to create a standard curve using the data from table 1. Consider the best way to graph this data. Make sure the type of graph that you select will allow you to add a line of best fit. Display the equation for the line of best fit directly on the graph. Hint... you'll want to think about how to treat the triplicate values when making the graph. Also consider the type of graph that should be selected to allow for addition of the line of…B ut of 1 e flag us page In Canada, facilities that participate in sterile compounding follow guidelines provided by USP Chapter , NAPRA and this professional association: Select one: O a. CAPT Q b. CPTEA O C. FDA O d. CSHP FS Q Search F6 - F7 F8 L F9 m |--- ■■■ 8 + Time left 21:19: F10 Next page3. Animation 12.1: This table shows results from allele-specific oligonucleotide hybridization analyses conducted on three different patients. The analyses all used the same probes constructed to test for the presence of a mutant gene. A plus sign (+) indicates hybridization occurred and a negative sign (-) indicates no hybridization occurred. Patient #1 Patient #2 Patient #3 Probe for normal allele Probe for mutant allele Which patient(s) is/are homozygous for the mutation and which patient(s) is/are heterozygous for the mutation? O Patients #2 and #3 are homozygous and Patient #1 is heterozygous for the mutation. O Patient #2 is homozygous and Patient #1 is heterozygous for the mutation. O Patient #1 is homozygous and Patients #2 and #3 are heterozygous for the mutation. O Patient # 1 is homozygous and Patient #2 is heterozygous for the mutation.
- 0.9kb dna fragment inserted into GEM1lr.colonies modt likely...... light blue blue white no colonies ......... used to confirm presence of 0.9 kb . dna sequencing Pcr microarray a and bWa... SLSU SVM Status... X ↓ O 650 O 550 O 500 200 O 850 Smal ↓ O 1550 Applicant Dashbo... 300 Msel 350 Probe Smal ↓ Caption: Restriction enzyme sites are indicated by the arrows. Smal binds to CCCGGG sequences. Msel binds to TTAA sequences. Enzyme 'X' refers to an unnamed restriction endonuclease that is not affected by DNA methylation. The distance between enzyme sites is indicated as number of bases. Assume only CpG methylation occurs. A human genomic segment (depicted above) was subjected to various experimental conditions (enzyme digestion, DNA methylation) AS INDICATED BELOW. 200 If the DNA was not methylated and digested with enzymes X, and Smal, what is the largest DNA fragment size that would show up on a Southern blot using the radiolabeled probe? Smal ↓ 500 MacBook Pro X ↓The main reason/reasons that motivated the geneticengineers to use E. coli in the production of monoclonal antibodies compared to the usual techniques is/are: O a. The quality of monoclonal antibody produced by the usual method is poor. O b. The establishment of hybridomas is not easy. O. Theusual technique produces only polyclonal antibodies. O d. The isolation of lymphocytes is difficult. O e. The isolation of spleen cells is difficult.
- I understand that microarrays are being used to define the molecular abnormality and the prognosis in some patients with leukaemia. What are microarrays?The figure below represents the size of various SSRS that are used for forensic analysis. The bars corresponding to each locus represent the range of size of the various alleles of the locus. 100 bp 200 bp 300 bp D8S1179 D21S11 D7S820 CSF1PO D3S1358 TH01 D13S317 D16S539 D2S1338 D199433 VWA TPOX D18S51 FGA D5S818 III Each color in the figure represents a set of molecular markers that can be analyzed simultaneously. Which of the following pairs of markers, which are not grouped together in the figure, could be analyzed simultaneously if they were the only two markers being studied? FGA and CSF1PO 400 bp THO and VWA THO and TPOX O TOPX and FGAExplain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.