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- Explain how acetaminopen gives such spectroscopic and chromatographic graph by interpreting the peaks that appeared in the graphs. Fig1. Mass Spectrometric Chromatogram of Acetaminophen (the two graphs) Fig2. IR Spectrograph of AcetaminophenIn kool aid chromatography, what factors affect the efficiency of the column? Also discuss the Van-Deemter equation.PART B: ANION TESTS Section 4: Test for Carbonate (CO ) ions. Your conclusion for each test will be that CO was present or absent in the sample tested. In the conclusion box write that CO was present or absent. OPERATION OBSERVATION CONCLUSION 6 M HCl with NazCO3 Bubbling 6 M HCl with NaCl Clear solution 6 M HCI with NazSO4 Clear solution 6 M HCl with Na;PO4 Clear solution 6 M HCI with unknown Clear solution Section 5: Test for Chloride (CI') ions After HNO; added AgNO3 with Na2CO; / HNO3 precipitate Clear solution AgNO, with NaCl / HNO, White precipitate Precipitate stays AGNO3 with NazSO4/ HNO3 Clear solution Clear solution AgNO, with NazPO4/ HNO; White precipitate Clear solution AGNO3 with unknown / HNO3 White precipitate Clear solution Section 6: Test for Sulfate (SO²') ions After HNO3 added BaCl2 with NazCO;/ HNO; White precipitate Clear solution BaCl2 with NaCl / HNO3 Clear solution Clear solution BaClz with Na,SO,/ HNO; White precipitate Precipitate stays BaCl2 with Na,PO4/ HNO,…
- For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm Rs when H = 3 cm > Rs when H = 5 cm Rs when H = 3 cm < Rs when H = 5 cm The two quantities cannot be compared due to insufficient information.The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
- What is the minimum volume of dissolution media required to provide sink condition in dissolution test of a tablet dosage form (its weight: 1350 mg; dose strength: 1000 mg) regarding the following conditions? The molecular weight of an active drug substance is 500 Da; the dissolution media is water and the solubility of active drug substance in water is 10 mg/Ml (4. SLAYT) 500 ml 1000 ml 1350 ml 100 ml 135 mlB. You will need approximately 1 ml of the 100 µg/ml bromophenol blue (which is your most concentrated standard) to generate your standard curve. Calculate how much bromophenol blue stock solution and how much water you will combine to accomplish this. Make 1 ml of this solution using a 100-1000 μl micropipetter. Use the equation C₁V1=C2V2 μl of 1 mg/ml stock + μl of water = 1 ml of 100 µg/ml bromophenol blueThis is a question regarding spectrophotometry. Scenario: 0.1 mL of the unknown and 0.9 mL of distilled water are mixed in a cuvette and the absorbance is measured at 280 nm. Given the absorbance, molecular mass and the extinction coefficient of the unknown are 0.253, 16950 g and 13940 M-1cm-1 respectively, and the path length is 1 cm, how can I find the concentration of the unknown in mg/mL by Beer-Lambert Law (NOT molarity M)?
- What is the iodine indicator test used for? Describe in detail how iodine works in a reaction and what results are producedResults and Discussion: Folin's McCarthy Sullivan Test: Samples: proline, methionine, alanine Reagents: sodium hydroxide (5N), Glycine (1%) and 10% sodium nitroprusside solution, 6N HCl -To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (5N), followed by addition of few drops of glycine (1%) and 10% sodium nitroprusside solution (HANDLE WITH CARE)and vortex. Place the test tube in a hot water bath, maintained at 40 C, for 15 minutes. Cool the test tube in ice cold water for 5 minutes and add 0.5 ml of 6N HCl. Vortex the contents and allow to stant fpr 15 minutes at room temperature.10 mL of 0.5 M CuSO₄ * 5H₂O solution from 10 M CuSO₄ * 5H₂O solution