3. Hexokinase catalyzes the first reaction in glycolysis and phosphorylates D-glucose to D-gluc 6-phosphate using ATP as the source of the phosphate. Under conditions of pH 7, 25°C and a Hexokinase concentration of 3.0 nmol/mL, the KM for Hexokinase for the substrate glucose was determined to be 3.0 x 10 M. a. When the glucose concentration was set to 160 μM, the initial rate of the reaction was found to be 65.0 μmol/(mL-s). What is Vmax for Hexokinase under these conditions? b. What is the turnover number for Hexokinase under these conditions? c. What is the catalytic efficiency for Hexokinase under these conditions?

3. Hexokinase catalyzes the first reaction in glycolysis and phosphorylates D-glucose to D-gluc 6-phosphate using ATP as the source of the phosphate. Under conditions of pH 7, 25°C and a Hexokinase concentration of 3.0 nmol/mL, the KM for Hexokinase for the substrate glucose was determined to be 3.0 x 10 M. a. When the glucose concentration was set to 160 μM, the initial rate of the reaction was found to be 65.0 μmol/(mL-s). What is Vmax for Hexokinase under these conditions? b. What is the turnover number for Hexokinase under these conditions? c. What is the catalytic efficiency for Hexokinase under these conditions?

Chemistry: Principles and Reactions

8th Edition

ISBN:9781305079373

Author:William L. Masterton, Cecile N. Hurley

Publisher:William L. Masterton, Cecile N. Hurley

Chapter23: Organic Polymers, Natural And Synthetic

Section: Chapter Questions

Problem 46QAP: Glycolysis is the process by which glucose is metabolized to lactic acid according to the equation...

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Calculate the equilibrium constant for the phosphorylation of glucose to glucose 6-phosphate at 37.0 °C. 4.74 x10¬3 M-1 eq In the rat hepatocyte, the physiological concentrations of glucose and P; are maintained at approximately 4.8 mM. What is the equilibrium concentration of glucose 6-phosphate (G6P) obtained by the direct phosphorylation of glucose by P;? 8.75 x10-8 [G6P] = M Incorrect
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For the triose phosphate catalyzed reaction: dihydroxyacetone phosphate <--> glyceraldehyde-3-phosphate the standard change in Gibbs free energy is ΔG'º=7.53 kJ/mol. Calculate the ΔG for this reaction at 298K when the concentration of dihydroxyacetone phosphate is 0.161 M and the concentration of glyceraldehyde-3-phosphate is 0.00163 M.
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