3. (a) (b) What are the different methods of cell lysis? Discuss in detail. In Streptomyces filtration from an erythromycin broth using a test filter, we find the following data: Filtration time, sec Volume of filtrate, L 5 10 20 30 0.040 0.055 0.080 0.095 The filter leaf has a total area of 0.009m² and the filtrate has a viscosity of 1.1 cP. The pressure drop is 0.51m of mercury and the feed contains 0.015 kg dry cake per liter. Determine the specific cake resistance a and the medium resistance RM.
Q: Identify the component of plasma membrane that allows it to carry out its function.
A: Introduction: The plasma membrane is made up of lipid layers as well as cholesterol. The thickness…
Q: Arrange the macromolecules in the order of priority of use as energy sources by cells fats…
A: Introduction Cells require energy for various metabolic activity. Energy is produced in the…
Q: Complexes of IgG bound to soluble multivalent antigens can activate the classical pathway on the…
A: Introduction :- One of three mechanisms that activate the complement system, a component of the…
Q: a. Sphingolipids always contain glycerol and fatty acids. b. Phosphatidylcholine is a typical…
A: Introduction :- The term "cerebrosides" refers to a class of monoglycosylceramide…
Q: Tell if the layer/tissue can be found in old roots or not : 1. epidermis 2. cortex 3. exodermis 4.…
A: Plants have unique organs that enable them to thrive and procreate in a wide variety of settings.…
Q: What sorts of data lead Richard Owen to propose that organisms were designed according to an…
A: Richard Owen was a naturalist and the biologist explained the nature of evolution before the…
Q: What would be the standard free energy change if FADH 2 is used to reduce O 2 to water? Standard…
A: Introduction : Electron transport chain is the series of electron carriers, enzyme and cytochrome…
Q: Can you specifically verify which one is canola oil, oleic acid, pork lard, lecithin, and lauric…
A: Lipids Lipids are defined as organic compounds that are strictly insoluble in water and are only…
Q: Does the refractory period limit the number of action potentials that a nerve cell can produce per…
A: Introduction :- The interval after an action potential is created during which the excitable cell is…
Q: The ff. are phospholipids, except: OA. plasmalogen O B. lecithin O c. cephalin O D. choline
A: Phospholipids constitutes the important class of lipids ,which forms a very important part of plasma…
Q: The ff. are phospholipids, except: OA. plasmalogen O B. lecithin O c. cephalin O D. choline
A: Phospholipids are a subclass of lipids that have two hydrophobic "tails" made of fatty acids…
Q: Which of the following is the distinguishing characteristic between fats and oils? O A. Identity of…
A: Given here are some statements regarding fats and oils and following is the distinguishing…
Q: True or False An organism which has attained homeostasis is at equilibrium with its external…
A: Homeostasis Homeostasis is defined as the ability of an organism to maintain and adjust its internal…
Q: Question 1 - Which dialysis tube(s) had little or no change in mass after the 24 hr period? Why?
A: In biology, homeostasis is the process of maintaining balance ( such as temperature, fluid balance…
Q: Question: Select the mood disorder in which diagnosis requires symptoms present for at least two…
A: Mood disorders can occur due to mental health illnesses. Here mood or emotional state is distorted…
Q: Discuss how recombinant DNA technology could be used to develop a diagnostic method for the novel…
A: Introduction The combining of DNA molecules from two distinct species is known as recombinant DNA…
Q: Dendritic cells take up, process or present antigen by all of the following routes except Select…
A: Introduction :- Professional antigen-presenting cells known as dendritic cells (DCs) are essential…
Q: Layers of the Epidermis (from deep to superficial) 500 Stratum basale, Stratum spinosum, Stratum…
A: The skin is made up of three layers. They are : epidermis-the superficial layer , dermis the middle…
Q: The diagram below represents the chloroplast in which photosynthesis takes place. 2.1.1 2.1.2 2.1.3…
A: Introduction : Photosynthesis is the process of preparing food in the presence of sunlight and…
Q: Transmission is: O A. conversion of amino acid to hydroxyl acid B. loss of ammonia from amino acids…
A: The amino acids are the monomeric units of proteins that are bounded together by peptide bonds.
Q: Make a plan for bio gas generation in the house or apartment with theoretical explanation. Should…
A: When bacteria break down organic materials (biomass) without oxygen, biogas is created. Anaerobic…
Q: An oxygen-storage protein present in muscle
A: Protein are the large biomolecule and they are macro molecule and they are composed of long amino…
Q: Describe the properties that enhance the resistance of endospores to the processes that usually kill…
A: The typical living cell might be referred to as a vegetative cell. With the exception of a few…
Q: Question:- Fill in the blank: Selaginella produces roots like other vascular plants, but it also…
A: Selaginella is a creeping plant with simple and scale-like leaves on the branching stem. The roots…
Q: The metallic salt of a high fatty acid: O A. inorganic salt OB. glycerin O C. soap OD. detergent 33
A: Soap It is a salt of a fatty acid. Soaps are mainly used as surfactants for washing, bathing and…
Q: Find the cause of the inference that the existence of many mitochondria increases the cell's energy…
A: The body's organelle responsible for creating energy is the mitochondria. Inside the cell, it…
Q: Muscle versus bone Venn diagram Drag each word or phrase to the part of the Venn diagram to which it…
A: Each organ system in the vertebrate's body (including humans) is assigned a set of functions. These…
Q: What is the oxygen requirement of Varicella-zoster virus (VZV)?
A: Introduction One of the nine known herpes viruses that infect people is human alphaherpesvirus 3,…
Q: Who discovered Varicella-zoster virus (VZV)?
A: Introduction A virus is an infectious microorganism made up of a protein-coated segment of nucleic…
Q: Describe the difference between the cytoplasm and cytosol.
A: Introduction: The cell is the basic unit of existence for all living creatures. The cytosol and…
Q: The proper order of the key events in urine formation. Filtration → reabsorption → secretion →…
A: The urinary system- consists of organs that have the main function of removing metabolic wastes, and…
Q: Please help me to describe the pesticide and antibiotic resistance and how they are related. This…
A: Organisms and their environments are inextricably linked and interdependent. Every alteration in the…
Q: What is mesoderm? Name an invertebrate with mesoderm.
A: Introduction During embryonic development the three germ layers, ectoderm, endoderm and mesoderm…
Q: Tell if these tissues/layers are present in secondary growth of a OLD/MATURE dicot root (bitter…
A: Dicots Dicots are the plants that bear a pair of leaves (cotyledons) in the embryos of their seeds.…
Q: Which of the following statements about Notch 1 is correct? (Select all that apply.) Select one or…
A: Concept used : Notch 1 gene Definitions required - Notch 1 gene : Notch one is a gene that…
Q: 4. Which of the following is a characteristic of the fibularis tertius muscle? A innervated by the…
A: Question 4. Which of the following is a characteristic of the fibularis tertius muscle? A innervated…
Q: Would mitochondrial transmembrane transport of a protein bound for the mitochondrial intermembrane…
A: Introduction: Translation is the process through which proteins are produced from mRNA. After the…
Q: Can antibiotic resistance increase the genetic diversity of microorganisms to its host aquatic…
A: Antibiotic resistance is the characteristic acquired by microbes in order to escape from the…
Q: Determine the term selectively permeable when membrane. referring to the plasma
A: Introduction The cell membrane, the nucleus, and the cytoplasm are the three components that make up…
Q: 3) At the completion of meiosis, how many sets of chromosomes does each daughter cell contain?…
A: The cell division is a cell cycle which is responsible for dividing the parent cell into daughter…
Q: The common metabolic pathway OA. glycolysis B. beta oxidation Oc. Kreb's cycle O D. glucogenesis
A: In the following section i will be discussing about common metabolic pathway. and which of the…
Q: Elucidate the regulation of gene expression in Cyanobacteria. The discussion should include a view…
A: Cyanobacteria are prokaryotic cells that are able to photosynthesize. They are an important groups…
Q: What is the expulsion of loose watery unformed stools
A: Loose watery stools can be due to diarrhea caused by bacteria (E. coli, Salmonella), virus such as…
Q: Describe What kingdoms consist of eukaryotes.
A: Lipids, proteins, nucleic acids, and carbohydrates are the main building blocks of both prokaryotes…
Q: Enlist the five factors that usually effect or influence the diffusion of substances in the body.
A: The chemicals' ability to diffuse through the membrane is influenced by a number of variables. These…
Q: Which of the following provides the second signal for T-cell activation? Select one: a.…
A: T cells are generated in the Thymus and are programmed to be specific for one particular foreign…
Q: Genetic variation _______. (Choose an answer.) 1. is created by the direct action of natural…
A: The variation in DNA between individuals and communities is known as genetic variation. Mutations…
Q: The lens of the vertebrate eye develops only after the head ectoderm comes in contact with the optic…
A: Eye development in vertebrate take place during the late phase of gastrulation. Eyes are the…
Q: Haptens Require carrier molecules to be immunogenic Interact with specific antibodies even if the…
A: Introduction :- A hapten is a material that can bind to a particular antibody but is not itself…
Q: Describe how the absence of the RNA polymerase enzyme affects the process.
A: RNA polymerase is an enzyme. It is used in the transcription process. The RNA polymerase binds to…
Step by step
Solved in 5 steps
- 1. a. Jimmy has tasked Carl with aliquoting 18 ul of distilled water into microcentrifuge tubes containing restriction enzyme. What size micropipettor should Carl use for the most accurate transfer? b. List the correct volumes for each of the pipettors in the image below. For the P10 provide the volume in ul and for P200 provide the volume in ul and ml. P10 P200 1 4 9. 2. You are given the restriction enzyme Trll with the recognition sequence 5'...TGA^TCA... 3' to cut DNA. a) Would this cut result in blunt or sticky ends? Explain your answer. b) Restriction Enzyme Pmel contains the recognition sequence 5' ...GTTT^AAAC... 3'. Which restriction enzyme would result in more cuts, Trll or Pmel? Explain your answer. 3. 1. You are doing a DNA extraction using a Wizard kit as the first step to prove Joe Goldberg is guilty of murdering either his girlfriend, her best friend, her brother or her neighbour. Your tube contains a pellet and a supernatant after adding cold 70% ethanol and…a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b. The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.continuous disc stack centrifuge is operated at 5000 rpm for separation of tukers' yeast. At a feed rate of 60 min21, thirty of the cells are recovered. For operation at constant centrifuge speed, solids recovery is inversely proportional to the flow rate. (a) What flow rate is required to achieve 90% cell recovery if the centrifuge speed is maintained at 5000rpm thi What operating speed is required to achieve 90% recovery at a feed rate of 60 min211).
- Choose the best combination of depyrogenation methods: 0.45 um filtration + 0.22 µm filtration OUV+0.45 um filtration 0.22 µm filtration + UV treatment 0.45 um filtration + distillation 0.22 µm filtration + distillationConsider the sterilization of a sodium gluconate production medium in the holding section of a continuous sterilizer. Assuming constant temperature, the specific death rate constant of the contaminant is 20 s-1 . If the average residence time in the holding section is 10 seconds, calculate the Del factor for the following and explain the results. i. For Pe = 400 ii. For Pe = 400, assuming plug flow iii. For Pe = 100 iv. For Pe = 100, assuming plug flowAfter filtering off the yeast in a difficult process, using a Celite pad to the filtrate which contains the reduced product from the ethylacetoacetate: sodium chloride solution is added. What is this process called and why is it added? IT ALT+FIN Prir Al T-FN-F10 (Mac)
- An amino acid of interest was produced in a fed-batch culture using glucose in the feed medium. The initial volume of the quasi-steady-state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, max = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/g a) Determine the volume of the culture at t = 10hb) Determine the concentration of glucose at t = 10 hc) determine the concentration and total mass of cells at t = 10 hd) If product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the concentration of product at t = 10h.Beans the 21kg Pit Bull needs to be put on IV fluids STAT! He is now 3% dehydrated and had 25ml of diarrhea. Calculate his fluid needs step by step using the standard 50 ml/kg/day. Calculate MR for 24 hours= Calculate HD for 24 hours= What is his CL for 24 hours= Put it all together to give me rate per hour- number. Round to the nearest whole On a standard 15 gtt/ml drip set what is his drip rate in seconds= gtt/?sec) (write as ?For a gradient system with a gradient of 5-90% in 50 min, flow 2 mL/min, column 100 x 4.6 mm i.d., the first peak elutes at 20 min, and the last peak elutes at 50 min. Calculate k* for this system. 2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.
- Covid-19 patients may develop acute pulmonary edema (fluid retention in the lungs), and furosemide is often used to treat it. Another potential complication of covid-19 is acute kidney damage. Possible causes include hypoxia, blood clots and inflammation. To evaluate a patient for kidney damage, a medical team took blood samples and placed a catheter to collect urine for 24 hrs. Here are some results: Plasma creatinine: 50 mg/100 mL plasma Urine creatinine: 40.8 mg/mL urine Urine production: 1.5 L in 24 hr 1. What was the patient's creatinine clearance in mL/min? (Creatinine clearance = excretion rate of creatinine/plasma concentration creatinine). Show your work.Covid-19 patients may develop acute pulmonary edema (fluid retention in the lungs), and furosemide is often used to treat it. Another potential complication of covid-19 is acute kidney damage. Possible causes include hypoxia, blood clots and inflammation. To evaluate a patient for kidney damage, a medical team took blood samples and placed a catheter to collect urine for 24 hrs. Here are some results: Plasma creatinine: 50 mg/100 mL plasma Urine creatinine: 40.8 mg/mL urine Urine production: 1.5 L in 24 hr 1. What is the patient's approximate GFR (in mL/min)?Covid-19 patients may develop acute pulmonary edema (fluid retention in the lungs), and furosemide is often used to treat it. Another potential complication of covid-19 is acute kidney damage. Possible causes include hypoxia, blood clots and inflammation. To evaluate a patient for kidney damage, a medical team took blood samples and placed a catheter to collect urine for 24 hrs. Here are some results: Plasma creatinine: 50 mg/100 mL plasma Urine creatinine: 40.8 mg/mL urine Urine production: 1.5 L in 24 hr 1. What is the patient's approximate GFR (in mL/min) 2. Is this a normal GFR (in units of mL/min)? Did the patient sustain kidney damage?