2. What is your smallest volume that you can comfortably measure with your P20 pipettor? Can you measure all of needed volumes (see table, especially the values for DI). If your answer is no, what can you do to solve the problem? Could you perhaps use a different final volume (V,)? Concentration of standard protein 2.0 mg/ml Volume of standard solution to be prepared needed V, (uL) Diluted concentration Volume of Volume of dilution needed solvent needed C; (mg/mL) V2 (uL) V-50-V (uL) 2.0 50 50 2.0 0.05 50 DI D2 D3 2.0 0.1 50 50 2.0 0.2 0.3 0.4 D4 2.0 2.0 50 50 D5 D6 2.0 0.5 50
2. What is your smallest volume that you can comfortably measure with your P20 pipettor? Can you measure all of needed volumes (see table, especially the values for DI). If your answer is no, what can you do to solve the problem? Could you perhaps use a different final volume (V,)? Concentration of standard protein 2.0 mg/ml Volume of standard solution to be prepared needed V, (uL) Diluted concentration Volume of Volume of dilution needed solvent needed C; (mg/mL) V2 (uL) V-50-V (uL) 2.0 50 50 2.0 0.05 50 DI D2 D3 2.0 0.1 50 50 2.0 0.2 0.3 0.4 D4 2.0 2.0 50 50 D5 D6 2.0 0.5 50
Chapter7: Statistical Data Treatment And Evaluation
Section: Chapter Questions
Problem 7.21QAP
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Transcribed Image Text:2. What is your smallest volume that you can comfortably measure with your P20 pipettor? Can you measure all of needed
volumes (see table, especially the values for DI). If your answer is no, what can you do to solve the problem? Could you
perhaps use a different final volume (V,)?
Concentration of standard
protein
2.0 mg/mL
Volume of
Diluted concentration
to be prepared
C; (mg/mL)
Volume of
Volume of
standard solution
dilution needed solvent needed
needed
V2 (uL)
V=50-V, (uL)
V, (uL)
2.0
50
50
2.0
0.05
0.1
0.2
DI
50
D2
2.0
50
2.0
D3
D4
50
2.0
0.3
50
D5
2.0
0.4
50
D6
2.0
0.5
50
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