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- Exercise 2B (optional) - Field of View and Estimation of Size Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4.5 4,500 um Scanning (40X): 1.8 mm x 100X/40X = mm = Low power (100X): FOV diameter = 1.8 mm = 1800 um High power (400X): 1.8 mm x 100X/400X = 0.45 mm = 450 um =Oal8 mm = 180 Oil immersion (1000X): 1.8 mm x 100x/1000X = um Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification FOV diam. um FOV diam. um length um length umExercise 1B – Using the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?Procedure 1 Magnification Light is refracted through two lenses to obtain magnification-the ocular lens and the objective lens. Magnifi- cation of the ocular lens is usually 10x.This means that if you were to view a slide with only the ocular lens, the image would be magnified 10 times. Magnification of the objective lenses varies, but typically is 4× for low power, 10× for medium power, and 40× for high power. (To verify that this is the case for your microscope, look at the side of the objective lens, which usually is labeled with its magnification.) Remember that oil immersion provides even greater magnification at 100×. When calculating the total magnification, you must multiply the magnification of the ocular lens (10) by the power of the objective lens. Fill in Table 3.1 to determine total magnification at each of the different objective lenses on your microscope. Remember that the magnification of the objective lens is usually printed on the side of the lens itself. ABLE 3.1…
- Question: Assume two Silver/Silver-Chloride electrodes are interfacing with human tissue using the Direct contact and Gel-filled contact approaches. In one paragraph, describe which method is the better technique to reduce motion artifacts and explain why.INSTRUCTION: EXPLAIN THOROUGHLY THE TWO THEORIES INVOLVED IN GRAM STAINING (CELL WALL AND LIPIDS THEORIES) IN RELATION TO THE TWO STATEMENTS BELOW. In THEORY OF CELL WALL, Gram-positive bacteria have heavy peptidoglycan, which helps decolorizer (alcohol) to dehydrate the gram-positive cell wall and traps the crystal violet complex inside the cell wall and maintains the purple color of crystal violet. In THEORY OF LIPIDS, Gram-negative bacteria have high lipid amount (10-15%) in the cell wall, which makes decolorizer (alcohol) easily to remove the crystal violet complex, and then colorless cell wall is stained with safranin and appears pink/red color.Practice problems: Do on folder paper. 1. You are observing a cell; total magnification 100X. It looks like about 7 cells will fit across the field of view's diameter. How large is this cell? Explain. 2. For the 2nd specimen, you are using a total magnification of 400X. It's harder to estimate this time, so you approximated that 12 cells would fit across the radius. Explain how you would find out the size of one cell.
- RESULTS for Exercise 4: Microscopic Measurements Name Date Calibration of Microscope: PART A 1 Division on the Ocular Micrometer corresponds to: Objective Scanning Low Power μη High Dry um Oil Immersion um Show calculations for each objective: Exercise 4 Microscopic Measurements 35nsert Format Arrange View Share Window Help Biology Lab Exam 1 Sp 21 目 125% v Collaborate Insert Table Chart Тext Shape Media Comment View Zoom Add Page 3. (1 point) Show the answer to the following question in correct significant figures. 10.219 + 3.12 = 4. (7 pts) True or False. Circle the correct answer. True or False. A blank used to zero of spectrophotometer always contains just distilled water. True or False. The mode is the most commonly occurring number in a data set. True or False. One milliliter (mL) equals 1,000,000 microliters (µL). True or False. Peroxidase activity increases when the enzyme is boiled True or False. Enzymes are carbohydrates. True or False. Denaturing involves an enzyme breaking down into individual amino acids. True or False. Hydroxlyamine is a competitive inhibitor of peroxidase. 5. (1 point) How many µL (microliters) are in 2.32 L? Show your work for full credit. 6. (1 pts) Convert 1,745 mm tọ Km. Show your work for full credit. MAR étv 9. MacBook AirTest: Quiz 5: X Cell membrane fl X = 9.1 Signaling Mol X * Question Completion Status: ard.com/webapps/assessment/take/launch.jsp?course_assessment_id=_407798_1&course_id=_156261_1&content_id=_12333940_1&step=null a. Increasing the interaction with water molecules O e. only making them less fluid QUESTION 6 Bb Microsoft Word O d. Cl- O e. Na+ Since membranes are selectively permeable, which of these would be able to get through without any help? O a. glucose O b. methionine O c. molecular oxygen (02) - O c. polar molecules in place of three fatty acids O d. a phosphate group in place of a carbon in glycerol O e. none of the above X Bb Serial Dilution We x Bb Molarity Workshe X QUESTION 7 Compared to a triglygeride, a phospholipid has a. a phosphate group and polar molecule in place of one fatty acid O b. phosphate groups in place of two fatty acids O X Click Save and Submit to save and submit. Click Save All Answers to save all answers. hp K 6000 DO 0.8 ● W **** | M MyOpe D DE ** ****…
- INSTRUCTION: Answer the question properly Do not copy in Google, plagiarize checker will be used. QUESTION: Give specific examples of the ff.: (5 each) a.) gram (+) cocci, b.) gram (-) cocci, c.) gram (+) bacilli, d.) gram (-) bacilli, e.) gram (-) curved rod, f.) gram (-) coccobacilli.Answer this ff question: 1. Explain the importance of system suitability on HPLC method of analysis. 2.In UV and visible spectrophotometry, the specimen is generally dissolved in a solvent, and determinations are made at room temperature using a path length of 1 cm. Give the most common solvents suitable for UV or visible spectrophotometry.Flectrochemical gradieuts cam be used for the following: a - ing cau be used All of the fllow Can b- export of waste products | C- trausmission of rerve impulses d- contraction of fibers e- synthesis of ATP