2. Calculate the following: a. you are asked to prepare 10 NA slants and 5 NA stab in big tubes. How much NA should you prepare if the indicated preparation for NA is as follows: 1. Suspend 28g of nutrient agar powder (CM0003B) in 1L of distilled water. 2. Mix and dissolve them completely. 3. Sterilize by autoclaving at 121°C for 15 minutes.
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- Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (1210C) for 15 minutes. Cool to 45-500C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes. 1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.1. The prescription for an oral solution reads “1 tbsp ac & hs x 10d.” What is the minimum (in mL) of solution should you dispense? 2. A nursing floor requires half liter of 50% isopropyl alcohol. How many mL of 70% isopropyl alcohol will be needed for compounding this order? 3. How may grams of pure dextrose is needed to prepare 100cc of D50W?3) With our 12.5 ug/mL solution, we will make serial dilutions with a dilution factor of 2. We want to make 1 mL of 1/2, 1/4, 1/8, 1/16, 1/32, and 1/64 dilutions. Draw a diagram describing how you will conduct the serial dilutions and insert it below. The 1/2 dilution (tube 2) will be made from the 12.5 ug/mL solution (tube 1). The 1/4 dilution (tube 3) will be made from the 1/2 dilution (tube 2L
- 1. compute for patient B result of his chloride analysis with the following data using mg/dL unit factor: 355/ mg/dL , Absorbance sample = 0.2148, Absorbance standard = 0.3017 a. 24.8 mg/L b. 25.27 mg/dL c. 2.7119 mmol/L d. 252.7 mg/dL 2. an adult patient suffering from debilitation is supected of calcium deficiency. His doctor requested for calcium anaylusis with the following data gathered standard known value is B: Absorabance is 0.2876. sample absorbance is 0.4291 compute for the calcium in mmol/L a. 53.6mmol/L b. 5.36 mmol/L c. 11.9 mmol/L d. 119mmol/L 3. calculate the value of sodium where the PREC solution has diclored. data gathered are the following: Absorbance of Reagent Blank = 0.3124 Absorbance of sample = 0.114, Absorbance of standard = 0.275 value is 150mmol/L a. 108.2 mmol/L b. 170.4 mmol/L c. 795.72 mmol/L d. 79.5 mmol/LThe physician orders Cipro oral suspension 250 mg bid for 3 days for a uti. You have Cipro 5g/100 mL. How many mL will you administer per dose?1. Answer the following questions based on the table: Pickling Vinegar Conc NaOH (M) 0.1005 Trial #1 Trial #2 Trail #3 Trial #4 Volume Vinegar (mL) 2.00 2.00 2.00 2.00 Initial Volume NaOH (mL) 0.32mL 0.22 1.02 0.44 Final Volume (mL) 24.25mL 24.20 25.00 24.45 Volume NaOH added (mL) 23.93mL 23.98mL 23.98mL 24.01mL a) In the titration of vinegar, a student uses 12.36 mL of NaOH(aq) (Molarity 0.100 M) to titrate a 2.00 mL sample of vinegar. If the student used lithium hydroxide (Molarity: 0.200 M) instead, what volume of lithium hydroxide would you expect them to use? Show balanced equations. b) A student performs titrations of equivalent amounts of HNO, (aq) with 0.1 M NaOH(aq). The volumes of NaOH(aq) needed to titrate are measured to be: 18.09 mL, 17.92 mL, 18.17 mL. Using % difference, does the student need to perform another titration or are their values within acceptable agreement? Explain why or why not?
- A patient takes Mucogel suspension at a daily dose of 10 mL t.i.d. and hs. How much magnesium hydroxide will Mrs A have taken after 5 days of compliant use of Mucogel? (Mucogel contains magnesium hydroxide 195 mg and dried aluminium hydroxide 220 mg/5 mL.)18. A prefilled syringe contains 50 mg of lido- caine hydrochloride per 5 mL of injec- tion. Express the percentage concentra- tion of lidocaine hydrochloride in the injection. 18. 1% w/v lidocaine hydrochloride3. Astelin nasal spray contains 0.1% azelastine hydrochloride and 400 µg/mL of benzalkonium chloride as a preservative. A container is capable of delivering 200 metered sprays of 0.137 mL each. The percent concentration of benzalkonium chloride in the preparation is ________ %, and this is equivalent to a ratio strength of 1:2500 w/v. True or False?
- 1. Why is necessary to remove fat and tendons from the heart sample? 2. Why is necessary to completely grind the beef? 3. Why is necessary to balance the homogenate tubes for centrifugation? 4. How do you prepare a 100mL of 0.1 M phosphate buffer? 5. From the anterior buffer, how do you make 100mL of 0.05 M? 6. Calculate the amount of ammonium sulfate necessary to get a 20% solution? 7. Which is the importance of dialysis?Demonstrate the procedure for arriving at the answer. A. It is necessary to carry out baths with Amitraz 12.5% in a batch of 25 patients. The product label says to dilute 1.5 ml of the product in 1 liter of water, for this there is a 50-liter water tank to prepare the product. Each patient corresponds to 1.2 L of the product diluted in water for an effective treatment. Calculate: a) total ml of amitraz needed to make the dilution in the water tank. b) ml of the product for each patient. c) Total ml of diluted Amitraz for the batch of patients. Answer: R: a) 75 ml. b) 1200 ml. c) 30.000 ml (Ctrl)-1. Determine the serial dilution used from the original water sample (100 mL) with 4.8 x 105 CFU asshown in the diagram.