2. Biuret Test Color of 3% CuSO4 solution: Sample casein alanine albumin gelatin distilled water 3. Molisch Test Color of Molisch reagent: Sample glutamic acid casein gelatin 4. Xanthoproteic Test Color of concentrated HNO3: Sample glutamic acid tyrosine casein albumin gelatin Color of original solution Color of original solution Color of original solution Color after NaOH/CuSO4 Color after Molisch Reagent Color after HNO3 Color at the interface Color after NH4OH
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Experiment
AMINO ACIDS AND PROTEINS
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- &. How many milldiners of a 2wv Lidocaine HCI stock selution in water should be used in preparing 30 ml of a 0.5% wiv Lidocaine HCI solution? . From the following formula for antificial tears, caleulate the quantity of each ingrodient roquired to prepare half donen of the 0ml containers. Poyviny alcohol 149 Povidone Chiorobutanol Serile sodum chioride soution a.%. s to 100 m How many milldiners of water should be added to 60 ml. of a 2% wiv agqueous solution to prepare a 1% wiv solution?1) Order i Ampicillin 250mg IVPB qsh. Add to 5omL of Dsul and infuse over 20 minutes. Supply : Ampicillin 1g vial Reconstitutionn Instructions:add 9.5mL 0f Sterile for a Cuncentratiun of 19/1omL. water How mL of the reconstituted medication will you mary add to the minibag? anial N set MA with a drop factor of 15glm using what is the Infusiom rate ! a m If an electronic pump is auaitable,uhat is the infusiir rate in mL/hr?Chemical tests See bottom of page 8 for instructions on how to complete this table. C. Biuret test Solution turins olive greeni Yeu ight Pur ple A. Iodine test B. Benedict's test Description of Solution turne Slohtion turns positive result Tube No. and (copy from Contents dark Purple or brovniornd asbiutrldeins Pp. 7-8) decp blue-blakk or tomoto of Short +/- Appearance blue (from p. 9) らish | +/- | Appearance blae Appearance +/- 1 - Orense 2 - Glucose Starch orunde Orange 3 - ders Brtelt blue blue blve bue 4 - alanine oransC- blue - 5. blue iout bue t orange Why was it important to have tube number 1 in each test? (Don't just say "because it's the control", and don't copy from the lab instructions-write out your own understanding of why and how that tube was used.)
- 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes and filtered through non-phosphorus filter paper. 1 mL of the filtrate was taken and 13 mL of distilled water, 4 mL of sulfomolybdic acid and 2 mL of dilute SnCl2 solution were added and mixed, and after waiting for 15 minutes, the absorbances of the obtained solutions against pure water at 520 nm were read. If the function of the calibration graph obtained with 0.5-2.5 mg/mL standard phosphorus solutions is y= 0.245x + 0.107 and the absorbance value of the serum sample is 0.109, what is the amount of phosphorus in the sample?Penicillin V Potassium Oral Solution 125 mg/5 ml. Disp. --ml. Sig. 5 ml q 6 h ATC × 10 days. How many milliliters of medicine should be dispensed? O 100 ml 200 ml O 125 ml O 150 ml Clear selectionQUESTION 3 An unknown protein was determined using the following method: Bradford method Reagents Stock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue G250 in a mixture consisting of 100ml of 85% phosphoric acid, 50ml of 95% ethanol and 50ml 1M NaOH. Store at 4°C until precipitation occurs, at which point it is discarded. Working Bradford reagent: Prepare fresh by diluting 10ml of stock Bradford reagent to 250ml with distilled water. Stock bovine serum albumin (BSA) solution (10mg/ml): Dissolve 0.2g BSA in 20ml distilled water. Working BSA concentration range: 0.5 – 1.25 mg/ml Method 1. Prepare the following test tubes: Table 1: Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Unknown Distilled H20 1.0 ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.8ml 0.25 mg/ml BSA 0.1 ml 0.5 mg/ml BSA 0.1 ml 0.75 mg/ml BSA 0.1 ml 1.0 mg/ml BSA 0.1 ml 1.25 mg/ml BSA 0.1ml Unknown 0.2ml protein Working 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml Bradford 2.…
- 3. Astelin nasal spray contains 0.1% azelastine hydrochloride and 400 µg/mL of benzalkonium chloride as a preservative. A container is capable of delivering 200 metered sprays of 0.137 mL each. The percent concentration of benzalkonium chloride in the preparation is ________ %, and this is equivalent to a ratio strength of 1:2500 w/v. True or False?ODA stesa smal 3. What volume of a 2.5x10-³ M stock solution is required to produce 50.00 mL of a dilute solution that is 1.0x10-³ M? 10:noitos2/1otontant ds.I HIVA Experiment 8 emotiona zobas dgi inominoqx3 unit2000Test tube Ascorbic acid stock (20 μg/mL) Citrate buffer Final Concentrations 1 0 μL 1000 μL 0.0 μg/mL 2 125 μL 875 μL 2.5 μg/mL 3 250 μL 750 μL 5.0 μg/mL 4 500 μL 500 μL 10.0 μg/mL 5 750 μL 250 μL 15.0 μg/mL 6 1000 μL 0 μL 20.0 μg/mL 7 1 mL of the fresh orange juice (20x diluted) 8 1 mL of the heated orange juice (20x diluted) 9 1 mL of the pre-citrus urine sample (5x diluted) 10 1 mL of the post-citrus urine sample (5x diluted) Plot a graph in which y-axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors. 1. StandardCurve Sample (μg) Absorbance (520 nm) 0.0 0.621 2.5…
- 3. Imagine that you inoculated 5 tubes of BGLB for each of three dilutions, and found a combination of positives of 4-4-2, which is not found on the MPN Index table. Your dilution series was 1 ml, 0.1 ml, and 0.01 ml. Estimate the MPN/100 ml value using the calculation found on the previous page.Apple puree was analyzed for petulin by HPLC-MS-MS after SPE clean-up. The procedure was 10.0g of puree + 10μl of a 10μg/ml solution of isotopically labeled petulin as internal standard were treated with 10.0ml of pectinase and acetic acid, centrifuged, and filtered. Four ml of the filtrate was passed through a SPE cartridge. The petulin was eluted with 2.0ml of ethyl acetate. The sample was evaporated to dryness and the residue dissolved in 1.0ml of the mobile phase. The analyte signal was 127 and the internal standard 197. Calculate the concentration of petulin in the sample in μg/g (RRF=1).