2+ ions 6.46 The following data show the binding of Mg2+ with a protein containing n equivalent sites: [Mg2+ ]total/HM [Mg2+]free/M 108 35 180 288 65 115 501 752 248 446 Apply the Scatchard plot to determine n and Ka. The protein concentration is 98 µM.
Q: How would the following enzymes speed up or slow down glycolysis? - high levels of Amp - high levels…
A: In glycolysis, a 6-carbon molecule of glucose-6-phosphate is broken down into 3-carbon pyruvate. It…
Q: Devise a solid-phase synthesis of the Ala-Leu-Val-Phe-Met, using Fmoc protection and the…
A: Solid phase, chemical synthesis of peptides, takes place from the C-terminal to the N-terminal side…
Q: Draw a diagram of the histidine side chain in the protonated and unprotonated states. Show the…
A: The four types of biological macromolecules are proteins, nucleic acids, carbohydrates and…
Q: In the graph below there are oxygen -dissociation curves. Assume that curve 3 corresponds with…
A: The y axis of the curve- Saturation represents percentage of haemoglobin bound/saturated with oxygen…
Q: Draw the oligopeptides' structure and provide the corresponding name for each oligopeptide 1.…
A: Pepetides are polymers of amino acids. Amino acids in a peptide are joined together via peptide…
Q: Many reactions in metabolism are thermodynamically unfavorable (ie-the reaction DOES NOT favor the…
A: The thermodynamically unfavorable reaction where ∆G°>0 is driven in metabolism by coupling with…
Q: What is the hydrogen bonding pattern within an alpha helix? a. Lone pair on C=O of residue i to…
A: We have to determine the hydrogen bonding pattern within an alpha helix
Q: Please draw the structure of the amino acid Histidine at each ionization state and please also give…
A: a) Histidine has three ionizable groups and equilibrium equations for its three ionizations are…
Q: Which of the following statements is not true about triacylglycerols? When hydrolyzed, they produce…
A: Triacylglycerols, often referred to as triglycerides, are a class of lipids that serve as a primary…
Q: Draw a diagram of a peptide bond in the cis and trans configurations. Show the contributing…
A: The amino group (NH2) of one amino acid and the carboxyl group (COOH) of another amino acid can join…
Q: Identify the applications for gel filtration chromatography. separation of components in a mixture…
A: Chromatography techniques are used to separate components of a mixture by the virtue of differences…
Q: Molly is a 10-year-old girl of short stature with hyperelastic skin which bruises easily, and…
A: 1. Amount of Vitamin C in Jane’s diet fails to improve Jane’s symptoms because the dermatosparaxis…
Q: In cytosol Enzyme 1 Enzyme 2 In mitochondria CH₂(CH₂) COOH A B C TI F F DE D E +ATP + PP₁
A: Beta-oxidation is the catabolic pathway by which fatty acids are catabolised into acetyl coA…
Q: Depicted above are the Ramachandran plots for Glycine and Alanine. Why do acceptable values for…
A: Peptide conformations are restricted by the dihedral angles phi and psi. The peptide bond has a…
Q: Draw a diagram of the hydrogen bonding interactions in a right-handed alpha helix (show all main…
A: There are four classes of biological macromolecules: proteins, nucleic acid, carbohydrates and…
Q: What pairs of sugars represents constitutional isomers, stereoisomers, epimers, enantionmers,…
A: Carbohydrates are polyhydroxy aldehydes or ketones. Monosaccharides are simplest carbohydrates:…
Q: 8. When oxygen binds to the heme group, by how many angstroms does the Fe2+ appear to shift? 9. How…
A: The heme group is a prosthetic group in oxygen-binding protein. Its primary function is to bind…
Q: After a polypeptide chain has been synthesized, certain amino acids in the peptide may become…
A: The modified amino acid shown in the structure you provided is serine (Ser).The modification that…
Q: to use the pH simulator to answer the question: . Select the Macro setting on the simulator…
A: Go to the pH simulator homepageOpt for the "Macro" mode on the simulator.Find the green pH meter and…
Q: Which foods are better at slowing down the rate of alochol absorption into the bloodstream?.
A: When alcohol is ingested, it is not digested and metabolised by our body. Instead, it enters the…
Q: Indicate the net charge of cysteine at pH 9.5
A: There are four classes of biological macromolecules- proteins, nucleic acids, lipids and…
Q: In lab 4, you calculated cholesterol levels of a sample directly based on the absorbance ratio of…
A: In laboratory experiments involving the quantification of cholesterol levels, precise and reliable…
Q: Match each error with its consequence for protein purification by column chromatography. Incorrect…
A: Column chromatography is a separation technique that uses a stationary phase and a mobile phase to…
Q: An Fab fragment binds to lysozyme with a dissociation constant of K4 = 10 M. A 1 nM (10 M) solution…
A: Consider the following reaction: where P is the protein, L is the ligand and k1 and k2 are rate…
Q: Which of the reactions are spontaneous (favorable)? L-malate + NAD+ oxaloacetate + NADH + H+ AG =…
A: Gibbs free energy change (ΔG) is a thermodynamic quantity that represents the maximum reversible…
Q: In order for ATP to effectively deliver energy to an enzyme, the following needs to be the case: a.…
A: Endergonic reactions are reactions that cannot be driven forward without an input of energy.…
Q: 7) The figure below shows a B-barrel protein embedded in a hydrophobic membrane. The central cavity…
A: Beta barrels are known to be embedded in the membrane for various functions like transport of cargo.…
Q: Select the choice that best describes the stereochemistry of the following amino acid, and rank the…
A: Cysteine is one of the 20 standard amino acids that serve as the building blocks of proteins. It is…
Q: Part C If, in addition to the constraints on glucose concentration listed previously, we have in the…
A: Pi + Glucose -> Glucose-6-Phosphate + H2OFor the above reaction, ΔG°’ = + 13.8 kJ/mol and…
Q: Consider the sterilization of a sodium gluconate production medium in the holding section of a…
A: Answer :- Sterilization is a critical process in various industries, including food and…
Q: A water molecule has polar O-H bonds that result in regions of partial positive charge (hydrogen…
A: Ion dipole interaction is defined as the force of interaction between charged ions and polar…
Q: Refer to the figure below, what are the following forces of attraction is involved in the protein…
A: The tertiary structure of the protein is a 3D structure established and stabilised by the…
Q: Construct the mechanism of a serine-protease with a catalytic triad.
A: A serine protease is an enzyme that cleaves peptide bonds in proteins and peptides. It possesses a…
Q: Which solution conditions would be best used for eluting a transcription factor from a DNA affinity…
A: We need to identify the solution conditions used for eluting a transcription factor from a DNA…
Q: Collagen is composed of the collagen triple helix containing 1042 amino acids, How long is the…
A: Structure of Collagen: Collagen is a major structural protein in the extracellular matrix. It is…
Q: 1 a- What is the approximate length of a DNA molecule (in the B form) containing 10,000 base pairs?…
A: The approximate length of a DNA molecule containing 10,000 base pairs can be calculated by using the…
Q: name from the following terpene, steroid, Prostaglandin, arachidonic acid, triglyceride, saturated…
A: Lipids are a diverse group of organic compounds that are essential to the structure and function of…
Q: Which of the following define the stereochemistry of alanine (as per the structure shown)? Note:…
A: Amino acids are biomolecules that are comprised of two functional groups, these are an amino group…
Q: Classify the inhibitor characteristics according to one of three types of inhibition: reversible…
A: 1.Reversible NoncompetitiveCharacteristic: Inhibitor binds noncovalently at other than active…
Q: Calculate the equilibrium constant K for the isomerization of glucose-1-phosphate to…
A: ∆G0 of the given isomerization reaction is -5.61 KJ/molGiven temperature = 298 K Then what will be…
Q: The tripeptide Glu-Pro-Arg (EPR) which is a product of Lactobacillus casei fermentation of milk was…
A: A peptide is a name assigned to short polymers of amino acids that are less than 50. Tripeptide is…
Q: Determine whether each of the amino acids is polar, nonpolar, positively charged, or negatively…
A: Amino acids are biomolecules that have an amino group and a carboxyl group linked to the same carbon…
Q: Put these three molecules in the order of most oxidized to least oxidized. нн нс-с-н нн H > > 0=…
A: In chemistry, the concept of oxidation state plays a crucial role in assessing the degree of…
Q: Consider an alpha-helix comprised of twelve amino acid residues. How many hydrogen bonds should be…
A: Proteins are linear polymers of amino acids which form the basic unit. Amino acids consist of a…
Q: Phosphofructokinase catalyzes the conversion of ... O A) oxaloacetate to citrate B) glucose to…
A: The process of breaking down glucose to generate energy is known as glycolysis. It produces two…
Q: Why is the net charge 0 for cooh? Shouldn't it be +1?
A: Amino acids are biomolecules that have an amino group, a carboxyl group and a side group that is…
Q: A receptor-ligand complex has a dissociation constant of Kd = 20 nM. The rate of receptor-ligand…
A: The reverse rate constant (k-1) is a parameter used in chemical kinetics to describe the rate at…
Q: Place each charge form of alanine under the pH condition where it would be the predominant form. The…
A: Alanine is a nonpolar amino acid. Amino acids are biomolecules that have an amino group, a carboxyl…
Q: Find the charge of the peptide KEALYR at pH 7.2. Below is the pKa chart. Provide your answer up to…
A: Before going into the intricate and precise calculation that gives us the charge of the given…
Q: Which statement about the glycoprotein shown below is true? OH CH₂OH OH HN HN-C O CH3 H₂ Protein CH…
A: Chemically, carbohydrates are polyhydroxy aldehydes or ketones. Monosaccharides are single unit…
Trending now
This is a popular solution!
Step by step
Solved in 4 steps with 5 images
- Quantitative Estimation of Amino Acids by Ninhydrin http://vlab.amrita.edu/?sub=3&brch=63&sim=156&cnt=2 can u help me with question 2 of the assignment questions Based on the experimental data provided, estimate the amount of amino acid in the given unknown solution by Ninhydrin method. SI No. Volume of standard amino acid solution (ml) Amount of amino acid (µg) OD at 570nm 1 Blank 0 2 0.2 0.12 3 0.4 0.25 4 0.6 0.45 5 0.8 0.55 6 1.0 100 0.68 7 Unknown (0.5ml) 0.41Protein concentration can readily be determined using the Beer-Lambert law: A = e l c where A = absorbance e = molar absorption coefficient (M-1cm-1) l = light path length (cm) c = concentration (M) If the molar absorption coefficient at 280 nm for yeast ADH is 48860 M-1cm-1 and a 10 mL solution of the protein has an absorbance at 280 nm of 0.4 (as measured by a spectrometer with pathlength 1 cm), then what is the concentration of the protein solution (in μM)? i.e. concentration = ______ μM If the molecular weight of the protein is 36849, what is its concentration in mg/mL? i.e. concentration = _______ mg/mL For each part of the question, show your calculations to arrive at your answers.80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pl. The solution is then titrated with 0.2M HCI to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCl needed to reach each relevant pKa value and equivalence point(s). Relevant pka values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.
- A tetrapeptide, glutamate-glycine-alanine-lysine, is prepared at at concentration of 1 mM (0.001 M) and is measured in the standard setup (pathlength of 1 cm). What is the approximate absorbance of this peptide at 280 nm? Hint: if the peptide contained a single tryptophan, the answer would be about 10. 10 280 1 013. 0.9% (m/v) NaCI solution and 5% (m/v) glucose solution are both isotonic to red blood cells. SHOW your work and watch sig figs & units. c. convert the concentration from M to % (m/v) for a 0.342 M NaC solution. (HINT: convert to g/ml and then multiple by 100%)Given the Ramachandran Plot below, identify the protein components that could adopt the phi-psi angle combination indicated by the number 3.
- A spherical cell with the diameter of 10uMhas a protein concentration of 20 mg/ml. Determine the number of protein molecules within the cell if the molecular weight of an average protein is 50,000 daltons (g/mol). Recall that Avogadro's number is NA 6.0221367×1023 molecules/mol. =A spherical cell with the diameter of 10uMhas a protein concentration of 20 mg/ml. Determine the number of protein molecules within the cell if the molecular weight of an average protein is 50,000 daltons (g/mol). Recall that Avogadro's number is N₁ = 6.0221367×1023 molecules/mol.A protein gives, under conditions of buffer composition, pH, and temperaturethat are close to physiological conditions, a molecular weight by size exclusion measurements of 140,000 g/mol. When the same protein is studiedby SDS gel electrophoresis in the absence or presence of the reducing agent β-mercaptoethanol (BME), the patterns seen, respectively, in lanes A and B are observed. Lane C contains standards of molecular weight indicated. From these data, describe the native protein, in terms of the kinds of subunits present, the stoichiometry of subunits, and the kinds of bonding(covalent, noncovalent) existing between subunits.
- a. An oligopeptide ALVGALGATPTPQMWSHSWRGVSIKS was digested with trypsin.Which method would be most appropriate for separating the products: ion exchange or gel filtration chromatography? Explain.b. Suppose that the peptide was digested with cyanogen bromide. What would be the optimal separation technique? ExplainIn a mixture of five proteins listed, draw an elution profile (Absorbance vs. mL eluted, arbitrary) for the purification of the listed proteins on a gel filtration chromatography resin: cytochrome c (pI = 5.4; Mr = 13,000), immunoglobulin G (pI = 7.3; Mr = 145,000), ribonuclease A (pI = 9.6; Mr = 13,700), RNA polymerase (pI = 6.3; Mr = 450,000), human serum albumin (pI = 5.4; Mr = 68,500). Label your elution peaks. Draw a sketch of an SDS-PAGE, reflecting the mobility of the above mixture as they elute from the column. Label you protein bands.A series of standard proteins and an unknown enzyme were studied by gel filtration on a Sephadex G200 (the 200 refers to the maximum pore size in kDa) column. The elution volume Vel for each protein is given in Table 1 below. (a) Plot the data in the form of log Mr versus elution volume. From the line of best fit through the points for the standards, determine the Mr of the unknown enzyme. Explain why ferritin and ovomucoid behave anomalously. Table 1 - The Vel versus Mr data Protein Mr Vel (mL) Blue dextran* Lysozyme Chymotrypsin Ovalbumin Serum albumin Aldolase Urease Ferritin# Ovomucoid# Unknown 1,000,000 14,000 25,000 45,000 68,500 150,000 500,000 700,000 28,000 ⎯ 85 200 190 170 150 125 90 92 160 139 *Blue dextran is not a protein but a high-Mr carbohydrate that has a covalently bound blue dye, and it elutes with the void volume of the column. # Do not…