1a)What is the purpose of adding 10mM of Tris-HCL (pH 7.5) during the extraction process? Oa. It breaks down the cell membrane to release the DNA. Ob. It ensures that the DNA that is released from the cell is turned into single stranded format. Oc. It neutralizes the the lysis reaction. Od. It denatures the proteins that were released from the cell.
1a)What is the purpose of adding 10mM of Tris-HCL (pH 7.5) during the extraction process?
Oa. It breaks down the cell membrane to release the DNA.
Ob. It ensures that the DNA that is released from the cell is turned into single stranded format.
Oc. It neutralizes the the lysis reaction.
Od. It denatures the proteins that were released from the cell.
1b) What is the purpose of the Phosphate Buffered Saline (PBS) in the DNA extraction protocol?
O a. The swab containing cheek cells is swirled around in this buffer to collect the cells.
O b. It is used to breakdown the cell membrane to release the DNA.
O c. It is used to denature proteins that were released from the cell.
Od. It is used as the main buffer in the PCR reaction.
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