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- 26. In a certain paper chromatography experiment, the Ri values of components X, Y, and Z are 0.25, 0.50, and 0.75. If the spot of component Z traveled 72 mm, the solvent front 26. would have traveled А. 18 mm В. 24 mm C. 54 mm D. 96 mm E. 216 mmwhich would stain with KMnO4 after a TLC Please provide only typed answer solution no handwritten solution needed allowedIt is not of the solute property detector used in HPLC is: a. Refractive index detector b. UV detector c. fluorescence detector d. UV-visible detector
- You have 100 mL of solvent available to do an extraction. Which will give a better separation? O A. 1 extraction with 100 mL B. 2 extractions with 50 mL C. 5 extractions with 20 mL O D.3 extractions with 25 mLWhat compounds present in a sample that can cause error in the analysis and alter desired results? A. Matrix B. Selectivity C. Analyte D. InterferenceA TLC analysis is performed on both crude and purified biphenyl. Upon visualization under the UV- lamp both spots remain at the baseline. This is likely because: a. Biphenyl is a polar molecule and therefore should remain at the baseline upon TLC plate development in the proper eluent. b. The samples were not spotted properly on the TLC plate. c. The solvent was too non-polar. d. The solvent was too polar. e. The solvent evaporated before the TLC plate could be added to the chamber.
- What is the probable cause of the sample streaking across the TLC plate from origin to solvent front.a. Too much sample b. Too little sample C. A developing solvent that is too polar d. A developing solvent that is too non polarWhich of the following statement is false? a. Component with the strong attraction to the support move more slowly than component with weak attraction. b. Chromatography technique used to separate only colored compounds. c. On TLC, single spot does not mean or guarantee a single substance (compound). d. Iodine vapor and UV- lamp are methods used for visualization uncolored compounds.I) Choose the Correct Answer from the Multiple-Choice List (10) 1. Which of the following is not a common type of packing for reversed-phase HPLC? C. Diol D. phenyl A. C18 B. C8 2. Gradient elution is a separation that employs : a. a single solvent of constant composition. b. solvent mixture of constant composition. c. two or more solvent systems that differ significantly in polarity. d. both (a) and (b). 3. The most useful bonded-phase coatings in HPLC are . : a) glass b) alumina c) inert silica d) siloxanes 4.Which of the following solvents is not commonly used in reversed-phase HPLC? a) Ethanol Water b) Hexane c) Acetonitrile d) Methanol e) water 5. It is not of the solute property detector used in HPLC is: a. Refractive index detector b. UV detector c. fluorescence detector d. UV-visible detector 6. High performance liquid chromatography (HPLC) can be used to : a. identify the various pigments from a leaf extract. b. separate types of organic pesticides. c. determine the mercury…
- QUESTION 15 To improve the accuracy of our linear fit between absorbance and concentration, a. add an extra data point to your linear regression where you make the assumption that pure water has an absorbance of zero. b. make sure that you choose a "Linear Regression" curve fit. c. ensure that there are no air bubbles in the cuvette. O d. use the data points only at the lower end of the concentration range because Beer's Law is not applicable for higher concentrations. QUESTION 16 What laboratory equipment do you need to prepare a 0.250 M stock solution of NaCI? O a. buret, graduated cylinder, analytical balance b. beaker, volumetric flask O. volumetric pipet, analytical balance d. analytical balance, volumetric flaskMatch Column A with Column B. v Distance traveled by the solvent A. Silica gel G Adsorbent used in TLC B. Eluent v High affinity to stationary phase C. High Rf value v High affinity to mobile phase D. Point of origin v Liquid that comes out of the column E. Low Rf value F. Solvent front G. Silica gel H. EluatePut all steps (below) of a TLC analysis in a correct order. Write the two main visualization methods as titles a. Developing the Plate b. Calculating Rf Values c. Spotting the TLC Plate Visualizing the Results e. Preparing the Sample f. Picking a Solvent