1. What is a “dNTP” and why are they included in the reaction mixture? 2. What will be the final concentration of 25 mM MgCl2, 5 mM dNTPs, and each 25 µM primer in one of the reaction tubes with the final volume of 50 microliters? Hint: use the information in the left column of Table 1 to help you with these calculations. (For one reaction) item 5.25x master mix – add things in order 32.7 µl ddH2O 171.7 µl 10 µl 5x reaction buffer 52.5 µl 3 µl 25 mM MgCl2 15.75 µl 0.5 µl 5 mM dNTPs 2.63 µl 1 µl 25 µM “forward” primer (#3012) 5.25 µl 1 µl 25 µM “reverse” primer (#3013) 5.25 µl 0.8 µl thermostable polymerase 4.2 µl (do not add until needed) Total: 49 µl Total: 257.3 µl

1. What is a “dNTP” and why are they included in the reaction mixture? 2. What will be the final concentration of 25 mM MgCl2, 5 mM dNTPs, and each 25 µM primer in one of the reaction tubes with the final volume of 50 microliters? Hint: use the information in the left column of Table 1 to help you with these calculations. (For one reaction) item 5.25x master mix – add things in order 32.7 µl ddH2O 171.7 µl 10 µl 5x reaction buffer 52.5 µl 3 µl 25 mM MgCl2 15.75 µl 0.5 µl 5 mM dNTPs 2.63 µl 1 µl 25 µM “forward” primer (#3012) 5.25 µl 1 µl 25 µM “reverse” primer (#3013) 5.25 µl 0.8 µl thermostable polymerase 4.2 µl (do not add until needed) Total: 49 µl Total: 257.3 µl

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter6: Oral Medication Labels And Dosage Calculation

Section: Chapter Questions

Problem 9.4P

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(For one reaction)	item	5.25x master mix – add things in order
32.7 µl	ddH2O	171.7 µl
10 µl	5x reaction buffer	52.5 µl
3 µl	25 mM MgCl2	15.75 µl
0.5 µl	5 mM dNTPs	2.63 µl
1 µl	25 µM “forward” primer (#3012)	5.25 µl
1 µl	25 µM “reverse” primer (#3013)	5.25 µl
0.8 µl	thermostable polymerase	4.2 µl (do not add until needed)
Total: 49 µl		Total: 257.3 µl