.What type of mutation is seen here? WT: 5′-AUG GCU AGA GUU GAA AAA-3′ Mutant: 5′-AUG GGU AGA GUU GAA AAA-3′ Group of answer choices a. Transition b. Tranversion c. Insertion d. Deletion
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1.What type of mutation is seen here?
WT: 5′-AUG GCU AGA GUU GAA AAA-3′
Mutant: 5′-AUG GGU AGA GUU GAA AAA-3′
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- 5 5 S 6 5 5 5 6 U 6 U 6 5:14 PM | 0.2KB/s HHHHH R R U RUUR ARU AP AP R U U R R AP R R R AP MOLECULAR...GENETICS. Describe gene regulation at transcription level. Explain the role of antsense RNA in control mechanism. Describe translational control mechanisms. Describe common DNA damages. Distinguish excision and mismatch repair. Describe the role of recA protein in recombination repair Elaborate on SOS repair mechanism. Define thymine dimer. How are they formed and repaired? Describe the molecular basis of mutation. 11 Leu+ Met+ Arg+ Write a detailed note on spontaneous mutation. Explain about mutant detection methods. Define reverse mutation. Describe the mechanism underlying Intragenic and intergenic suppressor mutations Describe the transposition mechanisms. 13 Vo LTE UNIT IV Time (Min) Describe the process of generalised transformation occurring in bacterial chromosome and plasmid. Elaborate on molecular mechanism and significance of transformation 22 Describe the process of…Which of the following concerning progeria are false? Choose all that apply. 1. Normally, Zmpste24 cuts pre-lamin to remove 50 amino acids 2. Exon 11 in LaminA codes for the binding site of Zmpste24 3. Progerin does not associate with the nuclear membrane due in part to the extra 18 amino acids it contains 4. The silent mutation known to cause Progeria results in a splicing defect affecting formation of mature LaminAAn experiment is carried out as depicted in Figure 1. Two drugs, staurosporine and camptothecin, are used to treat normal and cancer cells. Staurosporine is an inhibitor of protein kinases and can induce apoptosis by an undetermined mechanism. Camptothecin is known to inhibit the DNA topoisomerase type I. Discuss and conclude the effects of the drugs on the two different types of cells in this experiment. Normal cells Cancer cells 1. Add staurosporine 1. Add staurosporine Cells are arrested at restriction point Cells are arrested at restriction point 2. Add camptothecin 2. Add camptothecin Figure 1 Cells are arrested at restriction point Cells are killed when they pass through S phase 3. Remove both drugs Cell cycle resumes Dead cells
- a. What is your epigenome (i.e. epigenetics)? b. Does lifestyle affect your epigenome? Explain -c. Does your epigenome change with age? Explain d. What is epigenetic therapy? Is it working? Explain Edit View Insert Format Tools Table 12pt v Paragraph v в I Address DELL F7 F8 F9 F10 F1 F4 F5 F6 # % & 3 4 6. 7 8 9 Y ...Six strains of E. coli (mutants 1–6) that had one of thefollowing mutations (i–vi) affecting the lac operonwere isolated.i. deletion of lacYii. ocmutationiii. missense mutation in lacZiv. inversion of the lac operon (but not an inversion ofthe lacI gene)v. superrepressor mutationvi. inversion of lacZ, Y, and A but not lacI, P, oa. Which of these mutations would prevent the strainfrom utilizing lactose?b. The entire lac operon (including the lacI gene andits promoter) from each of the six E. coli strainswas cloned into a plasmid vector containing an ampicillin resistance gene. Each recombinant plasmidwas transformed into each of the six strains to create partial diploids. In analysis of these strains, mutant 1 was found to carry a deletion of lacY, so thisstrain corresponds to mutation i in the list above.Which of the other types of mutations would be expected to complement mutant 1 in these partial diploids so as to allow lactose utilization?c. In part (b), each strain was plated on…Four terms and statements related to gene regulation in bacteria are listed below. Match the correct term to each of the four statements: Regulatory molecule that binds to operator region in DNA. 1. repressor Regulatory molecule that must be present at a site in DNA (such as promoter) so that transcription occurs. 2. Constitutive 3. Effector Small molecules that bind to regulatory molecule, such as repressor. 4. Activator Phenotype of I+ oCz*
- 7) The protein VEGF plays an important role in stimulating the formation of new blood vessels. In some circumstances, cancer cells will produce too much VEGF. The drug PTC299 is currently being researched as a potential anti-cancer drug. It works by binding to VEGF mRNA, stopping this mRNA from being read by the ribosome. Therefore, not as much VEGF protein is produced. (National Cancer Institute. nd. NCI Drug Dictionary. Retrieved from: https://www.cancer.gov/publications/dictionaries/cancer-drug/def/vegf-inhibitor-ptc299) a) If approved, what kind of cancer treatment would it be considered (radiotherapy, gene therapy. anti-angiogenesis)? b) Briefly explain why you think PTC299 fits the kind of cancer treatment you indicated in a). Use information from the text to support your answer. c) Would PTC299 be considered a traditional or a targeted therapy?Normal DNA: TGC GTG CTT AAG CGG TGT ACA CGT TGC mRNA: Animo Acid: 1st Mutation TGC GTG CTT AAG CGA TGT ACA CGT TGC mRNA: Animo Acid: Do you think it will affect the protein’s function? Why? 2nd Mutation TGC GTG CTT AAG CGG TGT GCA CGT TGC mRNA: Animo Acid: What kind of mutation is this? Do you think it will affect the protein’s function? Why?1. Draw a diagram of the lac operon. PO I+P+O+Z+Y+ I+P+OcZ+Y- I-P+O+Z+Y+ Lab section (circle): lacz gene lac Y gene lacA gene I+P+O+ZY/IPO+Z+Y- 2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before actual mRNA production. Mutated promoters (P) cannot bind RNA polymerase, so no mRNA is made. In the table below, make predictions about the expression of ß-galactosidase and permease for different partial diploid (F') genotypes. Do this for E. coli growth on two different types of media - one without lactose, and one with. Use a to mark where there is no enzyme produced, and a + to mark where the enzyme is made. The first genotype has all wild-type alleles, and has been filled in as an example. Genotype IPOCZ+Y/I-P+O+Z-Y+ ISP+O+Z+Y/I+P+O+Z*Y* CR 1:00 Lactose absent B-galactosidase Permease R 3:-00 + R 6:30 Lactose present B-galactosidase F 10:00 Permease