Screw Cap Case Study

***Repeat steps 2-4 for each of the following: 5 mL of oil and 2 g each of cornstarch, sodium chloride, and sodium bicarbonate.

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Procedure: Filled each test tube with substances provided and subjected them to various conditions. These conditions included, heat, cold water, hot water, acid and basic additions and tested on litmus paper. The reactions were observed and documented at each step.

The first experiment begun by filling a 600-ml beaker, almost to the top, with water. Next, a 10-ml graduated cylinder was filled to the top with water. Once water was added to the beaker and graduated cylinder, a thumb was placed over the top of the graduated cylinder. This would ensure that no water was let out and no bubbles were let into the graduated cylinder. Next, it was turned upside down and fully submerged into the beaker. Then, a U-shaped glass tube was attained. The short end of the glass tube was placed into the beaker with the tip inside of the graduated cylinder. Next, a 50-ml Erlenmeyer flask was received. After, 10-ml of substrate concentration and 10-ml of catalase/buffer solution were placed into the flask. A rubber stopper was then placed on the opening of the flask. After adding these, the flask was held at the neck and spun softly

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The mixture was then transferred to a clean centrifuge tube via pipet, carefully not wetting the upper walls of the tube. Zinc granules were then added and the tube was immediately plugged with cotton 1/3 of the way into the tube. The tube was then warmed in a hot water bath for about 5 minutes, the folded red litmus paper was inserted at the top of the tube with a wet crease. After a few minutes, nitrate is indicated on the wet crease of the litmus paper, turning it blue. For the Carbonate test, 25 mg of carbonate sample was added to a centrifuge tube and 3 drops of 6 M H2SO4 was added. A disposable pipet was used to transfer a drop of Ba(OH)2, that hung directly from the pipet over the carbonate solution, and the observations of the drop were recorded.

First, each metal’s description and weight was recorded. Additionally, the weights of each cylinder used was recorded. Next, a graduated cylinder was filled with 20mL of water and unknown metal II was fully submerged in the water 3 times. Each time, the volumes were recorded and the difference between the final volume and initial volume was calculated. This test was repeated for unknown metal IV.

First, all of the equipment was obtained and placed on the table. The necessary equipment was weighed using a top loader balance, which used grams as its unit of measurement. The test tube, which held the samples, was filled with water from a plastic squirt bottle. A piece of filter paper, which had been folded several times, was placed over a funnel over an Erlenmeyer flask to filter the mixture in the test tube. The test tube mixture was poured onto the filter paper.

The pipette was used to transfer 8 mL of the 0.5 molarity solution into the graduated cylinder. Distilled water was added to raise the bottom of the meniscus to the 20.0 mL line and the solution was transferred into the beaker after it was rinsed with the solution. The pipette was used to take a small quantity of the solution and rinse and then fill a test tube with the solution. The amount of 0.2 molarity solution needed to create 20.0 mL of 0.1 molarity solution was calculated as 10.0 mL. The pipette was used to transfer 10.0 mL of 0.2 molarity solution into the graduated cylinder and distilled water added until the bottom of the meniscus reached the 20.0 mL line. The solution was transferred to the rinsed beaker and then a portion placed into a test tube that had been rinsed with the solution. The amount of 0.1 molarity solution required to create 20.0 mL of 0.05 molarity solution was calculated to be 10.0 mL. The pipette was used to transfer 10.0 mL of 0.2 molarity solution into the graduated cylinder and distilled water added until the bottom of the meniscus reached the 20.0 mL line. The solution was then placed into a beaker that had been rinsed with the solution and then into a rinsed test

The 20ml beaker was washed with dichloromethane to collect the solid left in the beaker. The filtered solution was collected in the 25ml filter flask. Then, the filtered solution was transferred to a clean centrifuge tube and the solid compound was transferred to a clean test

The samples were prepared in vials with one milliliter of methanol and one microliter of sample. Every sample is run under each condition three times with ten microliters being injected each time. The flow rate is set at one milliliter per minute and the temperature is maintained at 20oC. The log of retention times

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Firstly, in each of the boiling tubes place 8 ml of 2% starch solution using the syringe. Then in each of the test

An ice bath was prepared in a large beaker and a small cotton ball was obtained. 0.5 g of acetanilide, 0.9 g of NaBr, 3mL of ethanol and 2.5 mL acetic acid was measured and gathered into 50mL beakers. In a fume hood, the measured amounts of acetanilide, NaBr, ethanol and acetic acid were mixed in a 25mL Erlenmeyer flask with a stir bar. The flask was plugged with the cotton ball and placed in an ice bath on top of a stir plate. The stir feature was turned on a medium speed. 7mL of bleach was obtained and was slowly added to the stirring flask in the ice bath. Once all the bleach was added, stirring continued for another 2 minutes and then the flask was removed from the ice bath and left to warm up to room temperature. 0.8mL of saturated sodium thiosulfate solution and 0.5mL of NaOH solution were collected in small beakers. The two solutions were added to the flask at room temperature. The flask was gently stirred. Vacuum filtration was used to remove the crude product. The product was weighed and a melting point was taken. The crude product was placed into a clean 25mL Erlenmeyer flask. A large beaker with 50/50 ethanol/water

2cm of a solution was tested and added 2 cm of 10% of potassium hydroxide solution and the test tube was shaked.