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Genetic Engineering And Human Engineering

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Genetic engineering is a highly debated topic across the world right now as countries are split for and against genetically altering crops and livestock. The simple definition for genetic engineering according to CSIRO is “The use of modern biotechnology techniques to change genes of an organism, such as plant or animal.”(CSIRO, 2007)
The techniques or steps to genetic engineering are quite technical. The first stage of genetic engineering is to isolate the DNA from the organism. Once the DNA strand is separated it is cut by a restricting enzyme. The restricting enzymes snips the DNA into a smaller code usually between the G and C nucleotides. The restriction enzyme also leaves a unique sticky substance on the end of each new strand. The next step is too place the newly cut fragments inside a plasmid. A plasmid can be defined as “A linear or circular double-stranded DNA that is capable of replicating independently of the chromosomal DNA.” (Biology Online, 2009) this means in order for the new strands of DNA to be accepted by the cell it must be fixed inside a plasmid. The same restricting enzyme is used on the plasmid. This allows the DNA strand and plasmid to stick and join together. Once joined the phosphodiester bonds are formed by the DNA ligase on either edge of the strand. Transformation is the next stage as the newly formed plasmids are taken in by the host bacterial cells. The cells are now a DNA library and can be administered to the required organism.

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