What is the average radius of a piece of double-stranded DNA in water that has a link length of 3.9 nm and and is 2688570 links long? If the two strands of the double-stranded DNA are separated, each chain now has a radius of 608 nm. What is the link length for single-stranded DNA?
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- 0.5 104 10 102 10 1 10' 10² 103³ 10* Cot (mole × sec/L) In 1968, Britten and Kohne showed that once double-stranded DNA is dissociated into single- stranded pieces, the rate of reassociation of the complementary strands can be analyzed. For these kinds of experiments, the DNA is first sheared into fragments with an average size of several hundred base pairs. The fragments are dissociated (denatured) via heating, and then the sample temperature is lowered slowly while the reassociation is monitored. During reassociation, pieces of single-stranded molecules collide at random. If the pieces are complementary, a stable double-stranded molecule is formed. If no match is made, the pieces separate and continue encountering other single-stranded pieces. This process continues until all matches are made. a. The figure below shows an ideal time course for reassociation of DNA. Co is the initial concentration of DNA single strands in moles per liter. Time (t) of the reassociation is measured in…What is the complete base composition of a double stranded eukaryotic DNA that contains 24% cytosine?A duplex DNA molecule contains a random sequence of the four nucleotides with equal proportions of each. What is the average spacing between consecutive occurrences of the sequence 5'-ATGC-3'? Between consecutive occurrences of the sequence 5'-TACGGC-3'?
- What is the average radius of a piece of double-stranded DNA in water that has a link length of 5.5 nm and and is 3158738 links long? Submit Answer Tries 0/99 If the two strands of the double-stranded DNA are separated, each chain now has a radius of 723 nm. What is the link length for single-stranded DNA? Submit Answer Tries 0/99What is the axial ratio (length:diameter) of a DNA molecule 20 μm long?if the guanine content of certain segment of double-stranded DNA is 22% what is the adenine content?
- Draw the following structures and rate their relative solubilities in water (most soluble to least soluble): deoxyribose, guanine, phosphate. How are these solubilities consistent with the three-dimensional structure of double-stranded DNA?What is the complete base composition of a double- stranded eukaryotic DNA that contains 22% guanine?Given the sequence shown below, write the complementary DNA sequence, using the base-pairing rules, as well as the directionality of the strands: 5'- CGAGGCTAGGTTAACCTG-3'
- If a given double stranded DNA undergoes enzymatic hydrolysis targeting only the "a" side in the phosphodiester bond, what are the consequent nucleotide products of the hydrolysis of the 2 strands? (Please write the answers using only the letters corresponding to the bases with either the p or OH beside it to indicate the phosphate and OH groups respectively.) Sequence in one strand: TCGATCAGIf a given double stranded DNA undergoes enzymatic hydrolysis targeting only the "b" side in the phosphodiester bond, what are the consequent nucleotide products of the hydrolysis of the 2 strands? (Please write the answers using only the letters corresponding to the bases with either the p or OH beside it to indicate the phosphate and OH groups respectively.) Sequence in one strand: TCGATCAGSupercoiled DNA is slightly unwound compared to relaxed DNA and this enables it to assume a more compact structure with enhanced physical stability. Describe the enzymes that control the number of supercoils present in the E. coli chromosome. How much would you have to reduce the linking number to increase the number of supercoils by five?