The portions of the sample having the analyte with similar composition analyzed using the same procedure at the same time, termed: O matrix O representative O assay O replicates
Q: All three of these are gas chromatography runs from my sample. I'm trying to figure out what it…
A: To find the reason for varied retention times and intensities
Q: 8. Which solution is of known concentration that is used to react completely with an analyte? D.…
A: Standard solution is used to react completely with an analyte.
Q: A solution containing 2.30 mM of X (analyte) and 1.15 mM of S (standard) gave peak areas of 1728 and…
A: Given, known Amount of X = 2.30 mM, amount of S = 1.15 mM Peak area of X is 1728 and that of S is…
Q: Which solution is of known concentration that is used to react completely with an analyte?…
A:
Q: When spiking a sample with isopentyl alcohol, the isopentyl alcohol peak should increase in area on…
A: It is a calibration method that involves adding an additional amount of the substance of interest to…
Q: Which of the following is best to use to separate butanol from the volatile mixture? O a. Affinity…
A: chromatography used to separate butanol from the volatile mixture
Q: In Thin Layer Chromatography,why does the least polar of the analytes (biphenyl)have a higher Rf…
A:
Q: Which of the following detector NOT consider as solute property? O a. Conductivity О а. Ob. O b.…
A:
Q: solvent front origin
A: In thin layer chromatography the compounds separate based on polarity, the more polar compound move…
Q: Which of the following statements is .14 ?incorrect Number of theoretical plates decreases .with…
A: Solution
Q: Although we use pre-cast gels in this lab it is common practice to make your own polyacrylamide gels…
A: Note : Since you have posted multiple questions, we are entitled to answer the first only. Please…
Q: Which of the following statements about a gas chromatogram is correct? O The height of the peak in…
A: A multiple choice question based on Gas Chromatogram, which is to be accomplished.
Q: The chromatogram indicates that O MGDG eluted first from the column DGDG SGDG
A: The given chromatogram indicates the retention time of three components.
Q: Bradford protein assay. The tubes contained the following amounts of the BSA solution: 0, 20, 40,…
A: We know that, A = ϵcl Where, A = absorbance ϵ = molar absorptivity c = concentration l = path…
Q: A mixture of seven amino acids (glycine, glutamate, leucine, lysine, alanine, isoleucine, and…
A: Chromatography is the technique that is used to separate the amino acids based on the priority and…
Q: What type of components /mixtures can be separated by chromatography
A: Chromatography is a method which usually separate components of a homogeneous mixture based on the…
Q: In reverse phase chromatography which analyte will elute first, most polar or least polar?
A: In reverse phase chromatography, polar solvent is used as a mobile phase.
Q: Which of the following statements is true regarding liquid liquid extraction for sample's…
A: Liquid-liquid extraction is a technique used for the separation of analyte from a liquid mixture.…
Q: What would be a suitable blank for an assay where the samples contain protein in a solution with dye…
A: A question based on absorption that is to be accomplished.
Q: Consider the following setup of a capillary electrophoresis (CE) system: What are the two major…
A: Capillary electrophoresis is an analytical technique that separates ions due to their…
Q: How analytes are recovered from SFC and SFE
A: SFE is supercritical fluid extraction, in which components in a mixture are separated from each…
Q: what do the different colors in the result of paper chromatography experiment indicates? 2. Why do…
A: Paper chromatography is a technique in which colour chemicals or substances are separated.
Q: Analysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min…
A:
Q: Which compound elutes first and last in reverse chromatography
A: In reverse phase chromatography, stationary phase is non polar and mobile phase is polar in nature.…
Q: Question 5 In Gas Chromatography, there are no interactions between the solutes and the mobile…
A: True In the case of Gas Chromatography, the solute does not interact with the mobile phase because…
Q: How are analytes usually recovered after SFC or SFE?
A: Step 1 of 1 SFE is supercritical fluid extraction, in which components in a mixture are…
Q: Analysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min…
A:
Q: Biuret Assay accurately quantify protein concentration within the range of 5-150 mg/mL. JUSTIFY THE…
A:
Q: ▪ True or False: in a TOF analyzer, a fragment of low mass reaches the detector first ▪ In GCMS when…
A:
Q: Explain how we would run a paper chromatography on an amino acid sample (explain the procedure or…
A: Paper chromatography is the chromatography based on liquid-liquid partition chromatography. It…
Q: The reagent that is used in the preparation the sample and does not contain the analyte is called
A: The reagent blank is a solution that contains all the same components as in sample solution but no…
Q: Use the simulation lab to obtain the distance for the solvent front and the distance for each spot…
A: Rf value can be calculated using the expression: Rf=Distance travelled by the componentDistance…
Q: Analysis of an unknown plant extract yield two peaks X and Y in a chromatogram, 15.65 and 17.03 min…
A: Two peaks X and Y in a chromatogram yields in 15.65 and 17.03 min respectively in a 30 cm column.…
Q: how might chromatography be used to analyze the composition of the dye in a marker?
A: Chromatography is a method for analyzing mixtures by separating them into the chemicals from which…
Q: Using the Wijs Method, determine the Sample Weight of an unknown sample using the following data:…
A: iodine number is defined as the mass of iodine absorbed by 100 gm of sample the unsaturated fatty…
Q: would column chromatography separate ketone and alcohol
A: The detailed solution of the given question is solved below. COLUMN CHROMATOGRAPHY Column…
Q: Tim is interested in analyzing for Pyraclostrobin (pesticide) in strawberries using QuECHers method…
A: 1-10 mg of pesticide is on per gram of the fruitThe tim is slicing 500mg fruit i.e. its's possible…
Q: which of the following chromatographic methods is best used for separating amino acids?
A: Chromatography is the technique that is used for the separation of different components from the…
Q: An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl-sepharose…
A: Ion exchange chromatography is an analytical technique for separation of proteins on the basis of…
Q: If it was discovered that a polyaromatic hydrocarbon (PAH) having a density of 1 g/cm3 has a…
A:
Q: An ion chromatograph is used for the determination of chloride ions. Seven replicate asurements of…
A: Given: Number of replicates = 7 The replicate measurements obtained are 20, 21, 18, 22, 20, 22, and…
Q: Which of the following studies WILL LEAST LIKELY use the standard chromatogram of caffeine? analysis…
A: To determine: Which studies will least likely use the standard chrmatogram of Caffeien
Q: When the soft drink Jolt is used in place of Coke in the HPLC experiment, the concentration of…
A: The explanation is given below
Q: 35. The stationary phase, as well as mobile phase, are liquid in Adsorption column chromatography…
A: Answer: Chromatography is a separation technique, in which we separate the components of a mixture…
Q: What kind of chromatography would you for purification of full length antibody ?
A: Chromatography is used to seperate and purify mixture of species . Various type of chromatrographic…
Q: 1. Describe the different types of analytes which can be analyzed using the different methods of…
A: Two questions based on Beer-Lambert law, which are to be accomplished.
Q: 8. What properties cause the separation of amino acids in paper chromatography?
A: Different amino acids have different partition coefficient between water and organic solvent.…
Q: How do you weigh hydgroscopic samples?
A: Hydgroscopic samples contain moisture. Therefore it is measured in digital analytical balance.
Q: If two samples have identical Rf values, in a Paper Chromatography of Amino Acids lab procedure,…
A: The different amino acids can be separated by using the technique of Thin Layer Chromatography.…
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- A Sodium Chloride (0.1920g) NaCI was assayed using volhard Method, using 52mL of 0.0975N AGNO3 and 12.3 mL of 0.1192Ammonium thioscyanate. Calculate the sample in %.2) You are tasked with determining the barium content of a sample containing barium nitrate mixed with rubidium nitrate. The sample is weighed, dissolved into 50.0mg water, and treated with xs 0.500M sodium sulfate. The acid fully ionizes in this experiment. A white precipitate forms which is washed, filtered, and weighed multiple times, according to the data given below: Mass of sample 0.425g Mass of thoroughly dried filter paper 1.462g Mass of precipitate+filter after 1st drying 1.755g Mass of precipitate+filter after 2nd drying 1.699g Mass of precipitate+filter after 3rd drying 1.698g16) Which of the following(s) is/are correct? I- Selectivity factor is the amount of time a solute spends in the stationary phase relative to the time it spends in mobile phase II- The retention time for an analyte is the time interval between its injection onto a column and its appearance at the detector at the other end of the column. III- Distribution constant is the ratio of the total solute concentration in the stationary phase to that in the mobile phase. A) I, II and III B) Only II C) I and III D) II and III E) Only III for GC2
- 1.Which of the following correlations about the analyses of iodine and saponificationnumbers is/are incorrect?a. Alcoholic KOH: dissolve KOH to lipid sampleb. Hanus reagent: react with saturated lipid bondc. Reflux set-up: hasten reactiond. Blank titration: determine total mol of KOH2. A triacylglycerol (Sample X) was subjected to saponification analysis. 1.1 grams of thesample was refluxed with 5% alcoholic KOH and the refluxed solution was titrated with 17.2 mLof 0.44 M HCl titrant. The blank solution was titrated with 25.6 mL of the titrant.i. What is the saponification number of the sample? ii. Which of the following could be the identity of the sample? Show solutions pls a. Tripalmitin (MW: 807.3)b. Trilaurin (MW: 639.8)c. Triolein (MW: 885:4)d. Tristearin (MW: 891.48)5. Which term is INCORRECTLY defined? a. linear range - the range over which the response of an analytical method is proportional to analyte concentration b. dynamic range concentration range over which there is a measurable response to analyte, though the response is not linear c. blank solution - a solution containing all reagents and solvents used in the analysis to which no analyte is deliberately added d. standard solutions - solutions for which the standard deviation is known - 6. True or False: Internal standards correct random error caused by run-to-run variation. a. True b. FalseA student measures the following using a crude colorimeter. Intensity of blank =206 Intensity of solution = 168 What abosorbance value would the student report for the sample?
- In gravimetric analysis, volatile electrolyte is used in peptization step a. to form small nucleib. to easily dry precipitatec. to reprecipitate the analyteexplain please3. a) Briefly describe the advantage of using cardiac troponin I over cardiac troponin T in diagnosis of myocardial infarction (a heart attack). b) Enumerate five (05) features of an ideal tumour marker c) List five (05) components each for the following in a urine full report i. Macroscopic analysis of urine II. Microscopic analysis of urine 4. a) Briefly describe how the van den Burgh test is used in assessing serum bilirubin. b) List five (05) biochemical tests used in assessing renal /kidney function. c) Calculate the GFR based on the following biochemical data. 24-hour total urine volume = 1440 ml Serum creatinine concentration = 4 mg/dL == Urine creatinine concentration = 40mg/dL 3Is it possible for a single molecule to test true positive in all the qualitative assays? Why or why not?
- Calculate the water hardness (mg L-1 of CaCO3) (i.e. calculate the mass of CaCO3) please show working out. thank you :) Sample No. 1 2 3 4 Final Burette reading (mL) 24.40 46.90 24.70 47.20 Initial Burette reading (mL) 0.10 24.40 2.10 24.70 Final-Initial Titre (mL) 24.30 22.50 22.60 22.50In paper chromatography, a rectangular piece of filter paper serves as the mobile phase stationary phase eluent solute sample spotsQuestion 7 Which of the following statements is true regarding liquid liquid extraction for sample's preparation. O In liquid liquid extraction, the analyte is separated from the sample matrix using a chromatography stationary phase. The analyte sticks to the column and the water wash removes all species that do not adhere to the column. O Two solvents differe in their polarities are used where the analyte is extracted into the similar (close in polarity) solvent. The solid and liquid phase heterogenous mixture is centrefuged prior to sample measurement and analysis.