I purified my protein in the presence of 8 M urea. I have 25 mL of protein, and I need to reduce the concentration of urea to less than 5 nM. I place my protein solution in a dialysis bag and I place the bag in a beaker that contains 4 L of distilled water. How many rounds of dialysis would I need to perform to reduce the urea to less than 5 nM? Assume 4 L of distilled water each time.

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter11: Properties Of Solutions
Section: Chapter Questions
Problem 138CP: Specifications for lactated Ringers solution, which is used for intravenous (IV) injections, are as...
icon
Related questions
Question
how do you do this?
I purified my protein in the presence of 8 M urea. I have 25 mL of protein, and I need to
reduce the concentration of urea to less than 5 nM. I place my protein solution in a dialysis
bag and I place the bag in a beaker that contains 4 L of distilled water. How many rounds of
dialysis would I need to perform to reduce the urea to less than 5 nM? Assume 4 L of distilled
water each time.
Transcribed Image Text:I purified my protein in the presence of 8 M urea. I have 25 mL of protein, and I need to reduce the concentration of urea to less than 5 nM. I place my protein solution in a dialysis bag and I place the bag in a beaker that contains 4 L of distilled water. How many rounds of dialysis would I need to perform to reduce the urea to less than 5 nM? Assume 4 L of distilled water each time.
Expert Solution
steps

Step by step

Solved in 2 steps with 1 images

Blurred answer
Knowledge Booster
Tools in Analytical Chemistry
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry: An Atoms First Approach
Chemistry: An Atoms First Approach
Chemistry
ISBN:
9781305079243
Author:
Steven S. Zumdahl, Susan A. Zumdahl
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781133611097
Author:
Steven S. Zumdahl
Publisher:
Cengage Learning
Chemistry for Today: General, Organic, and Bioche…
Chemistry for Today: General, Organic, and Bioche…
Chemistry
ISBN:
9781305960060
Author:
Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:
Cengage Learning
Chemistry: The Molecular Science
Chemistry: The Molecular Science
Chemistry
ISBN:
9781285199047
Author:
John W. Moore, Conrad L. Stanitski
Publisher:
Cengage Learning
Chemistry by OpenStax (2015-05-04)
Chemistry by OpenStax (2015-05-04)
Chemistry
ISBN:
9781938168390
Author:
Klaus Theopold, Richard H Langley, Paul Flowers, William R. Robinson, Mark Blaser
Publisher:
OpenStax