Graphs Using Excel, create an absorbance vs. à curve for Brilliant Blue. Connect the points with a smooth curve. Create an absorbance vs. concentration (Beer's Law) graph for Brilliant Blue.

Chemistry for Engineering Students
3rd Edition
ISBN:9781285199023
Author:Lawrence S. Brown, Tom Holme
Publisher:Lawrence S. Brown, Tom Holme
Chapter1: Introduction To Chemistry
Section: Chapter Questions
Problem 1.77PAE
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Both images attached go together Also please calculate concentration
Graphs
Using Excel, create an absorbance vs. λ curve for Brilliant Blue. Connect the points with a
smooth curve. Create an absorbance vs. concentration (Beer's Law) graph for Brilliant Blue.
Transcribed Image Text:Graphs Using Excel, create an absorbance vs. λ curve for Brilliant Blue. Connect the points with a smooth curve. Create an absorbance vs. concentration (Beer's Law) graph for Brilliant Blue.
AnmAbsorbance
560 0.065 A
570 0.095A
580 0.104A
590 0.131 A
600 0.172 A
0.246A
620 0.357A
630 0.414A
640 0.338 A
610
650 0.181A
660 0.075A
Beer's law
Volume of Brilliant
Blue
Concentration of
Brilliant Blue
Absorbance at
amax
1.00mL
0.00000125 M 0.00000375M
0.135A
Purple Elution
Bond 1am = 500
3.00mL
Absorbance 0.420 A
Concentration
0.444A
5.00mL
0.00000625M 0.00000875M
0.670A
100mL
Bond 2nm = 600
Blue Elution
0.253 A
1.055A
Transcribed Image Text:AnmAbsorbance 560 0.065 A 570 0.095A 580 0.104A 590 0.131 A 600 0.172 A 0.246A 620 0.357A 630 0.414A 640 0.338 A 610 650 0.181A 660 0.075A Beer's law Volume of Brilliant Blue Concentration of Brilliant Blue Absorbance at amax 1.00mL 0.00000125 M 0.00000375M 0.135A Purple Elution Bond 1am = 500 3.00mL Absorbance 0.420 A Concentration 0.444A 5.00mL 0.00000625M 0.00000875M 0.670A 100mL Bond 2nm = 600 Blue Elution 0.253 A 1.055A
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