Consider a uniport system where a carrier protein transports an uncharged substance A across a cell membrane. Suppose that at a certain ratio of [A]inside to [A]outside, the AG for the transport of substance A from outside the cell to the inside, Aoutside Ainside, is -11.3 kJ/mol at 25°C.
Q: Please answer fast draw pyruvate carboxylase mechanism. cofsctors must include. label all…
A: Pyruvate carboxylase is an enzyme which catalyzes the conversion of pyruvate to carboxylase.…
Q: 12. The graph shows values of arterial plasma bicarbonate concentration and pH for different persons…
A: Metabolic alkalosis is caused by acid base imbalance in the blood. It is characterised by increase…
Q: The major source of metabolic acid in the body is carbon dioxide owing to its ability to be…
A: Given to us are 5 acids and we are asked to determine which one out of the 5 are produced from…
Q: Imidazole is used to elute His6-tagged proteins from Ni-NTA resins because imidazole is: a.…
A: On the Ni-NTA column the protein that is His-tagged bind to the column with high affinity. The…
Q: A.We have seen from history the role science has played in the prevention and control of diseases.…
A: Epidemiology : It is the study of diseases in a population, studying the reason , how it spread, why…
Q: 1. What is the superhelical density (o) of a closed-circular DNA with a length of 4,200 bp and a…
A: Supercoiling is the coiling of a coil. Now, whether the coil coils in a left-handed direction or…
Q: Elaborate on the industrial processes of water purification and wastewater treatment. In your…
A: Introduction Water pollution is process by which water get polluted for various human activities.…
Q: Water is present in the cell at a concentration of 55.5 M, whereas P, is present at a concentration…
A: Glyceraldehyde phosphate dehydrogenase is a 37 kDa enzyme which catalyzes sixth step of…
Q: Show the Dehydration Synthesis reaction that occurs to form a dipeptide containing the following…
A: Introduction: In the dehydration synthesis reaction, the carboxyl group of amino acid reacts with…
Q: Identify the type of glycosidic linkage present in the disaccharide below: O a (1-6) O a (1-4) 03(1…
A: Disaccharides are oligosaccharides composed of two monosaccharide units. The monosaccharide units in…
Q: In the alpha-helices, which of the following statement is NOT true? a. Backbone carbonyl…
A: Proteins are composed of amino acids that are attached together via peptide bonds. The linear chain…
Q: he write a conclusion and if the hypothesis was (rejected, modified, or supported) Questions: 1.…
A: Enzyme assays are laboratory tests undertaken to figure out the kinetics of an enzyme catalyzed…
Q: 1. The role of cofactors in the realization of the catalytic activity of enzymes.
A: Co-factor is the non-protein component that is required for an enzyme activity. Co-factors can be…
Q: What is the product of the oxidation of this fatty acid?
A: The aerobic fatty acid oxidation mechanism in the mitochondria converts a fatty acid into acetyl-CoA…
Q: Anaerobic pathways after glycolysis In the absence of oxygen, what anaerobic pathways can occur…
A: Introduction Glycolysis is a metabolic pathway where one glucose breaks and forms 2 molecules of…
Q: In the peptide, HAPPYNEWYEAR, there are a total of removed in its formation. (Write the symbol of…
A: A peptide is a polymer of amino acid residues linked via a peptide bond. A peptide bond is a…
Q: Give at least 10 functions of protein and describe each function. Cite an example for each function…
A: Proteins are the bio molecules which are very diverse in their structure and function. There are…
Q: 1. How does the resin facilitate purification in glutathione S-tranferase (GST) affinity? 2.…
A: In Biochemistry, affinity chromatography is a technique used for the purification of protein based…
Q: Food industry a. The importance of recombinant DNA technology in food industry b.…
A: Introduction Recombinant DNA technology (RDT) is a process by which DNA of an organism is altered in…
Q: What is the purpose of soaking the egg in vinegar? Explain the rationale of vinegar reaction to the…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Propose a pathway for the following compound to enter gluconeogenesis / glycolysis. In your pathway,…
A: Glycolysis is a collection of 10 enzymatically catalysed reactions that sequentially oxidise a…
Q: Which of the following single-stranded DNA molecules are likely to pair with each other to form…
A: DNA are polymers of nucleotides. A nucleotide consists of a nitrogenous base(A, T, G, C) attached to…
Q: In a 2017 study examining the efficacy and safety of its use in patients with mild-to-moderate…
A: The given drug has three ionizable groups. It is made up of the amino acid Lys, Pro, and Thr. The…
Q: is device used to measure temperature.
A: The device used to measure temperature is .........
Q: You've discovered a novel biosynthetic pathway that makes the molecule caffeine. The pathway can be…
A: In biochemistry, a metabolic pathway is series of enzyme catalysed reaction where product of one…
Q: Explain the role that Gla residues play in complexes. Explain the role of vitamin K / warfarins in…
A: Blood coagulation is a complex process where many factors are involved. This factors can be…
Q: How can chirality and stereoisomers influence the pharmacology, bioactivity, toxicology,…
A: Ibuprofen is a Non-Steroidal Anti-Inflammatory Drug (NSAID). Ibuprofen does this function by…
Q: Which of the following statements about alpha-helix and beta-sheet structure are true? More than one…
A: A protein's function depends on its structure. There are four levels of protein structure: primary,…
Q: Draw the structure(s) of the following amino acids (Asp, Lys, Phe, and Val) at pH 3.5, pH 6.5, and…
A: Amino acids are biomolecules that have an ionisable amino group and an ionisable carboxyl group…
Q: lon concentrations ION ECF K+ 4 mM Na+ 150 mM CI- 108 mM ICF 145 mM 12 mM 10 mM Eion = The table…
A: Resting membrane potential can be calculated by using the Nernst equation, if the membrane potential…
Q: Although phosphoesters are generally not energetic enough to donate the phosphate to make a high…
A: For an energetically favorable reaction, the change in free energy will be negative, and for an…
Q: If we were handed a tube of 2mg/mL BSA how much is required 20μL of each of the following…
A: Different concentrations of protein solutions are needed to be prepared during biochemical…
Q: Complex carbohydrates are comprised of which of the following? Disaccarides Oligosaccarides…
A: Carbohydrates are biomolecules composed of carbon, hydrogen, and oxygen. Carbohydrates are…
Q: Algae are being studied as a source of lipids to be used as source of biodiesel as a liquid fuel.…
A: Given that algae uses H2O, NH3, CO2 in the presence of light to produce a compound CH1.8O0.5N0.2 and…
Q: Draw the Citric acid cycle pathway and identify the different types of organic reaction mechanisms.…
A: Citric acid cycle involves 8 biochemical reactions that occur in a cyclic manner. The acetyl CoA…
Q: Discuss how the following buffer systems work in the human body: a) Carbonate buffer system b)…
A: All biological processes are pH dependent. Even a slight change in pH can result in a large change…
Q: Two compartments are separated by a semipermeable osmotic membrane through which only water…
A: Osmosis is the movement of water across the semipermeable membrane driven by the difference in…
Q: The Quikchange cycling parameters are listed below: 1.95 degrees, 2 min 2. 95 degrees, 20 sec…
A: PCR or Polymerase chain reaction is used in molecular biology to make multiple copies of…
Q: Human proteins are made up of ______________ (number of distinct/unique) amino acids.
A: Proteins are made up of amino acids attached together via peptide bonds. The proteins can fold up…
Q: Draw the Gluconeogenesis pathway (pyruvate to glucose) and identify the different types of organic…
A: Introduction: The term gluconeogenesis refers to the production of glucose or glycogen from…
Q: 15. Cellular proteins are oftentimes post-translationally modified. Choose one of the following…
A: Post translational modification are the modifications done in the protein by covalently attaching…
Q: Identify the components of animal fatty acid synthase (FAS). acyl-CoA dehydrogenase B-ketoacyl…
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: Sketch on one reaction rate vs. substrate concentration graph & sketch on one Lineweaver-Burk type…
A: LB plot is double reciprocal plot which gives Km and Vmax values as x intercept and y intercept…
Q: Using an arrow, draw the site of cleavage for the following peptides that are reacted by: Pepsin…
A: Amino acids are building blocks of peptides and polypeptide chain. Peptides are consist of 2-50…
Q: Which of the amino acids does NOT have an aliphatic side chain? a. Lysine b. Alanine…
A: R groups in aliphatics are nonpolar and hydrophobic. The hydrocarbon chain's hydrophobicity rises as…
Q: 384 Protein Purification Methods Q4.1- What chemical properties of sodium dodecyl sulfate (SDS)…
A: SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a type of electrophoresis…
Q: Intermediary metabolism includes all the reactions in an organism involved in generating and storing…
A: Metabolism is the total of all chemical transformation that takes place in a living cell. One…
Q: 7. Isocitrate dehydrogenase is inhibited by ADP and activated by ATP. T F
A: Isocitrate dehydrogenase catalyzes the oxidative decarboxylation of isocitrate to…
Q: what for is the acrolein test and the principle behind the test
A: Fats and oils are triacylgylcerols or made up glycerol attached to 3 fatty acids. There are several…
Q: Using an arrow, draw the site of cleavage for the following peptides that are reacted by: Pepsin…
A: Amino acids are building blocks of peptides and polypeptide chain. Peptides are consist of 2-50…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- Consider a uniport system where a carrier protein transports an uncharged substance A across a cell membrane. Suppose that at a certain ratio of [A]inside to [A]outside, the AG for the transport of substance A from outside the cell to the inside, Aoutside → Ainside, is -12.1 kJ/mol at 25°C. What is the ratio of the concentration of substance A inside the cell to the concentration outside? [A]inside [A]outside || 656275.63 Incorrect Choose the true statement about the transport of A under the conditions described. Increasing [A]outside will cause AG for movement of Aoutside to Ainside to become a smaller negative number. Decreasing the concentration of the uniport protein in the membrane will cause AG to become a larger negative number.A one-to-one protein (P)-ligand (L) complexation (P + L PL) has a dissociation equilibrium constant (Kd) value of 100 nM at 25°C, and the Kd remains the same at 37°C. 1) What is AS of binding at 25°C? Assume ACp of the binding is 0 over the temperature range. AS = 1.34E2 kJ/(mol*K) (note the unit!!) (sig. fig =3) 2) What is the concentration of the PL complex formed at equilibrium when you mix 0.20 uM (microM) of Protein and 1.0 uM of Ligand together at 37°C? PL at equilibrium = 8.1E-1 uM (note the unit!!) (sig. fig =2)The effect of temperature on the hydrolysis of lactose by a ß-galactosidase is shown below in Table 1. The temperature coefficient, Q10 is the factor by which the rate increases by raising the temperature 10°C. The universal gas constant, R is 8.314 J/mol.K. (a) (b) Table 1: Data of Vmax over temperature T (°C) 20 30 35 40 45 Vmax (umoles/min.mg protein) 4.50 8.65 11.80 15.96 21.36 Plot the graph of In Vm vs 1/T using any spreadsheet software (include all appropriate labels and equation). Calculate the activation energy Ea and temperature coefficient Q10.
- A bacterial enzyme catalyzes the hydrolysis of maltose as shown in the reaction given below: Maltose + H2O -> 2 glucose If the reaction has a Km of 0.135 mM and a V max of 65 m mol/min. What is the reaction velocity when the concentration of maltose is 1.0 mM?The acidity of the stomach is maintained by the H*/K* ATPase in parietal cells of the gastric mucosa. These cells have an internal pH = 7.4. The H*/K* ATPase transports H* across the cellular membrane into the stomach where pH = 0.8. (a) Given a membrane potential of 65 mV (inside negative), calculate AG in kJ/mol for the transport of H* into the stomach at 37 °C. Show your work. (b) The hydrolysis of ATP is directly coupled to H* transport and the stoichiometry of the transport reaction is such that 1 mol of ATP is hydrolyzed for every 1 mol of H* transported into the stomach. ATP is hydrolyzed by the ATPase on the inside of the cell. Under the conditions given in part (a) and assuming the steady-state concentrations of ATP, ADP and P₁ given below, is the transport of H* in to the stomach thermodynamically favorable at 37 °C? Show the calculation that supports your answer. Steady-state intracellular concentrations: ATP = 4.2 mM ADP = 360 μM Pi = 5.4 mM For ATP hydrolysis at 37 °C: ATP…Neutral sphingomyelinase 2 converts sphingomyelin into ceramide and phosphorcholine. What kind of enzyme is it? Assume Vmax is 35 µM min-1. When you provide 3.0 x 10-5 M of sphingomyelin, you observe an initial velocity of 6.0 µM min-1. Calculate the KM.
- An antibody binds to another protein with anequilibrium constant, K, of 5 × 109 M–1. When it binds toa second, related protein, it forms three fewer hydrogenbonds, reducing its binding affinity by 11.9 kJ/mole. Whatis the K for its binding to the second protein? (Free-energychange is related to the equilibrium constant by the equa-tion ΔG° = –2.3 RT log K, where R is 8.3 × 10–3 kJ/(mole K)and T is 310 K.)An experiment was carried out to measure the reaction rate of hydrolysis of acetylcholme (substrate) with serum enzymes (Eadie, 1949). In the experiment, two experiments were conducted, namely experiment 1 without using a prostigmine inhibitor and experiment 2 using a prostigmine inhibitor at 1.5 x 10^-7 mol/l. the data obtained are: a. Is prostigmine competitive or noncompetitive inhibitor? b. determine the value of km and rmax for the two experiments, compareA bacterial enzyme catalyzes the hydrolysis of maltose as shown in the reaction given below: Maltose + H2O -> 2 glucose If the reaction has a Km of 0.135 mM and a V max of 65 umol/min. What is the reaction velocity when the concentration of maltose is 1.0 mM? (Please take note of the units)
- Consider a suspension of particles (isoelectric point is at pH 6) in water at pH 2 and a NaCl concentration of 0.001 M. Describe how the strength of repulsion varies with the following changes, assuming all other conditions remain constant. Give a description (more than just increase or decrease) in terms of the effect on the double layer thickness and the zeta potential. (a) Change from 0.001 M NaCl to 0.1 M NaCl, (b) Change from pH = 2 to pH = 5.An enzyme has the following values: vmax's; 0.028 M s-' and 0.021 M s-' (I), and km's; 0.00198 M and 0.00197 M (I), when the total concentration of enzyme is 10-º M. The (I) values were determined with 1 microM inhibitor. Select the value closest to the k, for this enzyme. (hint 1 microM = 10-6 M).The equation of the double reciprocal plot is y = 0.5294 x + 1.4960. What is the value of vmax (in M/s)? The substrate concentration is given in units of molarity (M) and reaction velocity has units of molarity per second (M/s). (Report to three significant figures)