Calculate the exact concentration of the stock protein solution in (mg / mL) based on the exact mass of albumin and the volume of the vial used to prepare the broth.     0.4905 g of albumin in 500 mL volumetric flask (mass of protein used to prepare the stock

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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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1. Calculate the exact concentration of the stock protein solution in (mg / mL) based on the exact mass of albumin and the volume of the vial used to prepare the broth.

 

 

0.4905 g of albumin in 500 mL volumetric flask (mass of protein used to prepare the stock 

Concentration of stock protein solution (mg/mL): 0,4905
Unknown number:
Labquest-SpectroVis instrument number:
Standard
Concentration
Concentration of
Diluted Unknown
Sample
Absorbance Corrected Absorbance*
(mg/mL)
(mg/mL)
Blank
0.)3)
0.338
0.568
0.892
0.994
1138
0.421
0.466
0.438
Standard 1
Standard 2
0.337
0.761
0,863
1.007
0,290
0,335
0,307
Standard 3
Standard 4
Standard 5
Unknown
Unknown
Unknown
1st replicate
2nd replicate
3rd replicate
Corrected absorbance is (Absorbance - Blank Absorbance).
Transcribed Image Text:Concentration of stock protein solution (mg/mL): 0,4905 Unknown number: Labquest-SpectroVis instrument number: Standard Concentration Concentration of Diluted Unknown Sample Absorbance Corrected Absorbance* (mg/mL) (mg/mL) Blank 0.)3) 0.338 0.568 0.892 0.994 1138 0.421 0.466 0.438 Standard 1 Standard 2 0.337 0.761 0,863 1.007 0,290 0,335 0,307 Standard 3 Standard 4 Standard 5 Unknown Unknown Unknown 1st replicate 2nd replicate 3rd replicate Corrected absorbance is (Absorbance - Blank Absorbance).
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