1. The chromatography solvent is very polar as it contains alcohol, an acid and water. Based on this information, list all the polar amino acids and arrange them from most polar to least polar.
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1. The chromatography solvent is very polar as it contains alcohol, an acid and water. Based on this information, list all the polar amino acids and arrange them from most polar to least polar.
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- 10. (a) A student performed a thin layer chromatographic separation experiment. In the first run, four standard substances A - azobenzene, B - benzoin, C -cinnamic acid, and D – dextrose were spotted on the plate. The line indicates the solvent front. In the second TLC run a mixture (X) of two of these same substances was separated. Based on Rf values, what is the identity of the unknown mixture X? (b) A second mixture, Y, was run at the same time. What is wrong with the solvent system used to try to separate Y? АвсDAn amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography.The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.
- Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?What is the purpose of: A. The concentrated salt solution? B. adding isopropyl alcohol to the solution? Why does it need to be in low temperature?In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 5 cm away from the finish line. The distance travelled from the starting line to finish line is 30 cm. The first amino acid, AA1 travelled 4 cm behind the distance travelled by the alcohol. The second amino acid, AA2 travelled 12 cm short from the solvent front while the third amino acid, AA3 travelled 4 cm ahead of AA2. a) Reproduce the chromatogram. Label. b) Calculate: Rf of AA1 ____ Rf of AA2____ Rf of AA3____
- o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the stock solution?1. How do you prepare 25 mL of a 0.4X solution of SDS from a 20X stock?2. How do you prepare 50 mL of a 0.25X solution of Trisethylenediaminetetraacetic acid (TE) buffer from a 100X stock?20. According to the package insert, adding 2.5 mL of sterile water to a 1 g cefazolin vial yields a final volume of 3 mL. What volume of this solution would you need to obtain 200 mg of cefazolin?
- 1. What is the concentration of the solution prepared by diluting 50 µl of ampicillin (1.0mg/ml) to a final volume of 1ml? 2. Determine the volumes of phenol, chloroform, and isoamyl alcohol required to prepare250 ml of a 25:24:1 (v/v) solution.You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?5. RX: Peppermint spirit 2ml Alcohol 10ml Sterile water QS 100ml Approximately how many fluid ounces of sterile water are needed to compound this solution?