See the Light week 2
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University of Nebraska, Lincoln *
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110L
Subject
Chemistry
Date
May 13, 2024
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5
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Experiment 5
See the Light – Week 2
Total Points: 78
Student’s Name: Parker Gould
Lab Section: 613
Report
Your report should include the following:
●
Data tables 5-4 and example calculations, 5-5, 5-6, 5-8 and example calculations, 5-9 and
example calculations
●
Absorption spectrum for each mixture (Red-Yellow, Red-Blue, Yellow-Blue, and all three
dyes) (5pts/graphs)
●
Absorption spectrum for unknown (5 pts)
●
Answers to questions
NOTE: You must show your work for all calculations; no work, no credit.
DATA TABLE 5-4.
Concentration of Mixtures of Known Concentration (4 pts)
Mixture
Red #40
(M)
Yellow #5
(M)
Blue #2
(M)
Red-Yellow
1.51x10^-
4
1.405x10^
-4
0
Red-Blue
1.51x10^-
4
0
1.61x10^-4
Yellow-Blue
0
1.405x10^
-4
1.073x10^-
4
All three dyes
1.007x10
^-4
9.367x10^
-5
1.073x10^-
4
Measured in SpectroVis (known mixtures only)
Example calculations must include all concentration calculations for the Red dye
(Red-Yellow, Red-Blue, and all three dyes)
(6 pts):
M1V1=M2V2
Red-Yellow
(3.02x10^-4)(10mL)=(M2)(20mL)
0.00302=M2(20mL)
0.00302/20
M2=1.51x10^-4
Red-Blue
(3.02x10^-4)(10mL)=(M2)(20mL)
0.00302/20
M2= 1.51x10^-4
All 3 dyes
(3.02x10^-4)(10mL)=(M2)(30mL)
0.00302=(M2)(30mL)
Experiment 5
See the Light – Week 2
Total Points: 78
0.00302/30
M2= 1.007x10^-4
DATA TABLE 5-5.
Absorbances for Diluted Stock Solutions and Mixtures (5 pts)
Dye
Analytical
Wavelength
(nm)
Red
Yellow
Blue
Red-Ye
llow
Red-B
lue
Yellow-
Blue
All
Three
Dyes
Red
#40
539.30
0.375
0.078
0.267
0.237
0.305
0.175
0.190
Yellow
#5
426.10
0.255
0.509
0.136
0.369
0.199
0.344
0.295
Blue
#2
610.30
-0.03
0
0.078
0.551
0.023
0.167
0.337
0.119
DATA TABLE 5-6.
Unknown (3 pts)
Dye
Present in Unknown
(circle)
Analytical
Wavelength (nm)
Absorption
Red #40
Yes
No
539.30
0.305
Yellow #5
Yes
No
426.10
0.075
Blue #2
Yes
No
610.30
0.123
DATA TABLE 5-8.
Concentrations in Unknown (6 pts)
Dye
Concentration (M)
Red #40
2.318x10^-4
Yellow
#5
5.343x10^-5
Blue #2
1.000x10^-4
(3 pts) Calculated using Beer’s Law
ε from Table 5-7
A from Table 5-6
Experiment 5
See the Light – Week 2
Total Points: 78
Example calculation must include concentration calculations for all dyes in the unknown.
(3 pts)
A=Ebc
Red
0.305=(1315.7)(1)(c)
0.305/1315.7=
2.318x10^-4
Yellow
0.075=(1403.7)(1)(c)
0.075/1403.7
5.343x10^-5
Blue
0.123=(1229.5)(1)(c)
0.123/1229.5
1.000x10^-4
DATA TABLE 5-9.
Concentration in Mixtures from Absorbance and Percent Error from Known
(10 pts)
Mixture
Dye
Known
Concentration (M)
(Calculated by dilutions
of diluted stock solutions)
Calculated
Concentration (M)
(Calculated from Beer’s
Law)
Percent Error
Red-Yellow
Red
1.51x10^-4
1.801x10^-4
19.27%
Yellow
1.405x10^-4
2.629x10^-4
87.12%
Red-Blue
Red
1.51x10^-4
2.318x10^-4
53.51%
Blue
1.61x10^-4
1.358x10^-4
-15.65%
Yellow-Blue
Yellow
1.405x10^-4
2.451x10^-4
74.45%
Blue
1.61x10^-4
2.741x10^-4
70.25%
All Three
Red
1.007x10^-4
1.444x10^-4
43.40%
Yellow
9.367x10^-5
2.012x10^-4
114.83%
Blue
1.073x10^-4
9.679x10^-5
-9.790%
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